Grants

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High-End Instrumentation (HEI) Grant Program (S10 Clinical Trial Not Allowed)

National Institutes of Health

High-End Instrumentation (HEI) Grant Program (S10 Clinical Trial Not Allowed)

2027-06-01

general

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High-End Instrumentation (HEI) Grant Program (S10 Clinical Trial Not Allowed)

National Institutes of Health

The High-End Instrumentation (HEI) Grant Program encourages applications from groups of NIH-supported investigators to purchase or upgrade a single item of high-end, specialized, commercially available instruments or integrated systems. The minimum award is $750,001. There is no maximum price limit for the instrument; however, the maximum award is $2,000,000. Instruments supported include, but are not limited to, nuclear magnetic resonance spectrometers, X-ray diffractometers, mass spectrometers, high throughput robotic screening systems, DNA and protein sequencers, biosensors, electron and light microscopes, flow cytometers, and biomedical imagers.

2027-06-01

Healthhealthcare

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High-energy human milk diets in the first two weeks after birth to reduce the severity of respiratory morbidity in extremely preterm infants

NHLBI - National Heart Lung and Blood Institute

PROJECT SUMMARY This hypothesis-driven, patient-oriented pilot randomized clinical trial will evaluate whether providing a high- energy human milk diet during the first two weeks after birth reduces respiratory morbidity in extremely preterm (EPT) infants. EPT infants, born at 28 weeks of gestation or earlier, have immature lungs and a high risk of developing bronchopulmonary dysplasia (BPD)—a chronic lung disease that increases mortality and long-term developmental complications. Early enteral nutrition is critical for lung development, yet most EPT infants receive insufficient energy during the first 14 days after birth. Docosahexaenoic acid (DHA) and arachidonic acid (ARA) supplementation is a promising strategy to increase energy intake through human milk, but there is no consensus on whether fat- enriched or protein-enriched diets are more beneficial for improving lung function. Building on findings from our recent NIH-funded trial, which demonstrated that protein-enriched diets improved growth and potentially reduced BPD severity, we will now assess whether a DHA/ARA-enriched exclusive human milk diet can achieve superior benefits. In this trial, we will randomize 150 EPT infants to receive either an exclusive human milk diet enriched with a commercially available DHA/ARA supplement or a standard, exclusive human milk diet during the first 14 days after birth. The primary outcome is the severity of respiratory morbidity at term equivalent age. We will also evaluate the feasibility of using non-invasive oscillometry to measure lung function in this population. This study will inform the design of a larger clinical trial and may establish that early enteral energy provision— unlike parenteral nutrition—provides measurable respiratory benefits. The findings could reshape neonatal nutrition strategies and optimize care for preterm infants at risk of BPD.

Up to $181K

2029-03-31

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High-performance energy systems

European Commission - EDF

High-performance energy systems. Call: Development actions implemented via actual cost grants. Programme: EDF. Open call from the EU Funding & Tenders Portal.

$50K

2026-09-29

clean_energy

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High-performance, circular-by-design, bio-based thermosets

European Commission - HORIZON

High-performance, circular-by-design, bio-based thermosets. Call: HORIZON-JU-CBE-2026. Programme: HORIZON. Keywords: Composites (including laminates, reinforced plastics, cermets, combined natural and synthetic fibre fabrics filled composites), Materials engineering (biomaterials, metals, ceramics, polymers, composites, etc.), New materials: oxides, alloys, composite, organic-inorganic hybrid, nanoparticles, Polymers and plastics. Open call from the EU Funding & Tenders Portal.

$50K

2026-09-22

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High-Priority Research in Tobacco Regulatory Science (Clinical Trial Optional)

upcoming

National Institutes of Health

High-Priority Research in Tobacco Regulatory Science (Clinical Trial Optional)

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High-Resolution In Vivo MicroCT Imaging to Advance Translational Musculoskeletal and Multidisciplinary Research

OD - NIH Office of the Director

We request support for the acquisition of the Bruker SkyScan 1276 CMOS Edition High-Resolution In Vivo MicroCT System to serve a large, diverse, and NIH-funded research community at the University of Rochester School of Medicine and Dentistry (URSMD). The instrument will be housed in the Biomechanics and Multimodal Tissue Imaging (BMTI) Core of the Center for Musculoskeletal Research (CMSR), which supports over 60 NIH-funded investigators across 12 departments. The SkyScan 1276 will replace an obsolete 21-year- old scanner and expand our capabilities in longitudinal and cross-sectional imaging of murine and larger animal models. The SkyScan 1276 provides true spatial resolution down to 6 µm, with voxel sizes as low as 2.8 µm, and enables high-throughput imaging with helical scanning, slip-ring gantry, and physiological monitoring. It supports a large field of view (75 mm × 310 mm), allowing full-body imaging of small animals and complex 3D constructs in a single scan. The SkyScan’s robust software suite will ensure reproducible, high- quality data analysis in alignment with ASBMR standards. These capabilities are vital for a broad range of applications, including skeletal biology, tumor progression, vascular imaging, implant osseointegration, and regenerative medicine. This shared instrument will be accessible to NIH-funded investigators in the CMSR, Wilmot Cancer Institute, Aab Cardiovascular Research Institute, Department of Oral and Craniofacial Sciences, Allergy/Immunology and Rheumatology unit, and Environmental Health Sciences Center. Collectively, these groups have ongoing projects funded by P30, P50, R01, and U grants that require the SkyScan’s resolution, throughput, and physiological imaging features. Major users collectively project over 1,600 annual usage hours, representing 83% of estimated available time. The system will be operated by a dedicated and experienced technician and overseen by a seasoned core director and a previous S10 recipient. The core will be supervised by an internal Advisory Oversight Committee comprised of intuitional leaders and experts. Training plans include on-site and vendor-led education, annual workshops, and on-demand hands-on sessions. The acquisition of the SkyScan 1276 will transform imaging capabilities at URSMD by enabling precise, minimally invasive, and translational research. This investment will significantly enhance productivity, innovation, and impact across multiple NIH-funded programs and catalyze new discoveries at the interface of basic science and clinical translation.

Up to $466K

2027-05-14

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High-resolution phenotyping of the hemodynamic consequences of thoracic aortic aneurysm repair

NHLBI - National Heart Lung and Blood Institute

PROJECT SUMMARY In adults with descending thoracic aortic aneurysm (TAA), vascular repair (grafting) reduces the ~20% yearly rate of rupture and/or mortality but is associated with negative perioperative consequences. Currently, thoracic endovascular aortic repair (TEVAR) has become the standard treatment for descending TAA due its lower in and out of hospital morbidity and mortality. While repair is essential, there are known negative consequences of TEVAR related to the graft material’s stiffness. Notably, the non-physiological properties of the graft may attenuate the long-term benefit of TAA repair by reducing end-organ function. Indeed, age-related stiffening of the native aorta is known to increase arterial pressure, wave reflections and the associated adverse end-organ remodeling related to greater transmission of pressure and flow pulsatility in key pressure and flow sensitive organs, such as the heart, brain, and kidneys. Despite these well-known effects of native aortic stiffening, no study has comprehensively and directly assessed the hemodynamic consequences of descending TAA repair (i.e. stiffening) locally on the aorta or on the heart, brain and kidneys. It is known that placement of grafts in the ascending aorta elicits increased aortic flow velocity, local aortic stiffness, downstream descending aortic dilation, increased wall shear stress, and likely increases pressure and flow pulsatility, arterial wave reflections and aortic pressure that, together, elicit pathological vascular and end-organ remodeling associated with reductions in function and end-organ damage. Importantly, no study has performed high-resolution assessments of the hemodynamic (pressure, wave reflection and pulsatility) and end-organ consequences of descending TEVAR at rest, but also importantly not during exercise where the consequences of aortic grafting may be exaggerated. Thus, the overall aim of the K99/R00 proposal is to perform high-resolution phenotyping of the central hemodynamic consequences of TAA pre- and post-TEVAR at rest and during exercise and determine the magnitude of associated effects on end-organ structure and function. Our project will begin to fill the large unmet gap in understanding aortic and aortic aneurysmal physiology at rest and during exercise, the impact of the standard treatment for descending TAA, and point the way towards better recommendations in practice and for design of future therapies. Furthermore, these data will encourage medical device companies to design more compliant grafts so that the positive effects of aortic grafting in terms of reducing aortic rupture risk are not offset by inducing reductions in cardiac, brain and renal function. Completion of the proposed project will provide me with a unique skillset, different from that of my mentoring team, rendering me highly competitive for future NIH funding related to the study of hemodynamics and end-organ function in health and disease.

Up to $143K

2028-03-31

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High-resolution structural analysis of native amphotericin megasynthases

NCCIH - National Center for Complementary and Integrative Health

Project Summary. Polyketide natural products have provided critical therapeutics, and they will remain an important component of new drug discovery and manufacturing. This program aims to develop new technologies to enable the high resolution cryo-EM structural elucidation of Type I polyketide synthases (PKSs) isolated directly from natural producer organisms. To date these megasynthase proteins have proven challenging for structural biology studies due to significant technical challenges, including extremely large molecular weights, difficult heterologous expression and highly dynamic structural features. As such, very few structures have been reported, and most are at low resolution and do not represent full synthases. We have recently developed tools for dual site-selective crosslinking of PKSs that enforces dynamic constraint and has led to the collection of high- resolution cryo-EM structures in single-modular Type I PKSs. Here we propose the development of two key tools. The first will convert native holo-PKSs to their cognate apo- forms to enable crosslinking within native PKSs. The second is the development protein isolation methodologies for very large megasynthases (500 kDa to 2 MDa) from within native producer proteomes, such that the resulting isolated forms can be structurally elucidated by cryo-EM. The amphotericin synthase pathway from producer bacterium Streptomyces nodosus will serve as a model system for high-resolution structural analysis. Together these tools will offer a fundamental step-change in access to molecular detail of PKS megasynthase structure.

Up to $440K

2028-03-31

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High-Risk Research in Biological Anthropology and Archaeology

U.S. National Science Foundation

High-Risk Research in Biological Anthropology and Archaeology

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High-Risk Research in Biological Anthropology and Archaeology

U.S. National Science Foundation

Anthropological research may be conducted under unusual circumstances, often in distant locations. As a result the ability to conduct potentially important research may hinge on factors that are impossible to assess from a distance and some projects with potentially great payoffs may face difficulties in securing funding. This program gives small awards that provide investigators with the opportunity to assess the feasibility of an anthropological research project. It is required that the proposed activity be clearly high risk in nature. The information gathered may then be used as the basis for preparing a more fully developed research program. Investigators must contact the cognizant NSF Program Director before submitting an HRRBAA proposal. This will facilitate determining whether the proposed work is appropriate for HRRBAA support.

Up to $35K

rolling

sciencetechnology

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High-throughput and Machine Learning Optimization of Fluorescent Sensors for Multiplexed Imaging in vivo

NIMH - National Institute of Mental Health

PROJECT SUMMARY / ABSTRACT Monitoring neuronal activity modulation is pivotal for elucidating brain functionality and addressing neurological disorders. Despite the advancements brought by green fluorescent calcium indicators like GCaMP and neuromodulator sensors, a considerable gap persists in the development of red fluorescent sensors that match the properties of their green counterparts. This gap, characterized by limitations in dynamic range, photostability, and kinetics, restricts a more comprehensive exploration of neuronal interactions, especially in multiplexed, dual-imaging imaging scenarios. Additionally, the iterative engineering approach for new sensor development is notoriously slow and labor-intensive. Our central goal is to leverage our sensor screening platform, Opto-MASS, as well as our recent successes in using machine learning to expedite the optimization of fluorescent sensors. This project aims to engineer red fluorescent calcium and neuromodulator sensors that match the kinetics and dynamic range of green sensors and further enhance their properties. Our objectives include the rigorous benchmarking of these sensors against the best-in-class for properties such as dynamic range, kinetics, and photostability, followed by comprehensive in vivo validation across multiple laboratories and application scenarios using fiber photometry and two and 3-photon imaging. Our project is innovative because it utilizes a high-throughput screening assay capable of evaluating over 10,000 sensor variants from library collections in under an hour, a significant advancement over current methods. Coupled with pioneering machine learning models that identify key residues affecting sensor performance, we will significantly accelerate fluorescent sensor development, particularly for red calcium and GPCR-based sensors. Importantly, we aim to achieve these goals while reducing time and resource commitments. Our project directly addresses critical needs outlined in this FOA, including a broader range of reliable sensors in neuroscience research that facilitate nuanced, multidimensional studies of brain activity. By developing sensors with improved dynamic ranges, kinetics, and photostability, we aim to overcome existing barriers to multiplexed imaging of neuronal dynamics in vivo. Ultimately, the successful completion of this project would not only fill a vital gap in neuroscientific research tools but also align with the NIH BRAIN Initiative's objectives to advance neurotechnology and set new standards for molecular tool development and in vivo validation in neuroscience.

Up to $2.3M

2030-12-31

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High-Throughput Automated Patch Clamp Instrument

OD - NIH Office of the Director

PROJECT SUMMARY Ion channels control the membrane potential and electrical properties of a myriad of excitable cells, making them critical components required for the physiological function of numerous organ systems including heart, brain, smooth muscle and skeletal muscle. The function of these channels and resulting electrical properties of the membrane are often evaluated using patch-clamp electrophysiology. However, as the number of variables underlying channel function has grown with increasing knowledge of normal and disease states, conventional manual patch-clamp methods have become increasingly insufficient to support the research. At the University of Maryland, Baltimore (UMB) our faculty includes a broad group of researchers focused on understanding the normal and pathophysiological mechanisms of ion channels and membrane electrical properties. The goal of this proposal is to support those scientists in their study of the electrophysiological characteristic of excitable cells, enabling enhanced mechanistic understanding of ion channel function and disease, and facilitating pharmacologic and therapeutic studies aimed at targeting ion channel function for therapeutic effect. This will be achieved through the purchase of an automated patch-clamp system, the Nanion Technologies SyncroPatch 384. The capability of the instrument to simultaneously patch up to 384 cells will dramatically increase the throughput of biophysical studies currently in process at UMB, enabling new resolution of genotype correlation with electrical phenotypes and promoting the development of new therapies. The Nanion SyncroPatch 384 is capable of recording in either voltage-clamp or current-clamp mode from a wide array of cell types, with data quality comparable to manual patch-clamp techniques, but with significantly greater throughput and reduced reliance on specialized user skills. The instrument will comprise the University of Maryland High -Throughput Ion Channel (HTIC) core and will be housed in the Department of Pharmacology and Physiology, where multiple faculty with electrophysiological expertise are available to support the instrument. Strong financial, administrative and facilities support will be provided by the University to ensure the success of the instrument, which will support a varied array of NIH-funded research projects. In summary, the SyncroPatch 384 offers significant advantages over conventional manual patch clamp techniques, including higher throughput, greater consistency and reproducibility, advanced temperature control, comprehensive data acquisition and analysis, versatility, and access to patch clamp techniques for a broader array of researchers.

Up to $750K

2027-04-30

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High-throughput Characterization of Genetic Mechanisms of Traditional and Paired Prime Editing

NHGRI - National Human Genome Research Institute

Abstract The development of prime editing has allowed for genome manipulation with user-defined insertions, deletions, or single-nucleotide conversions, without installing harmful double-stranded breaks in the genome.5 This has revolutionized the genomic medicine field, with companies like Prime Medicine, Inc. now using prime editing in human clinical trials. Despite its versatility, prime editing often results in lower editing efficiencies for larger edits, and the nuances of how insertion and deletion edits are installed by prime editors are not well understood. 5,6,7,8 Past genomic mechanistic studies of prime editing have identified DNA mismatch repair (MMR) as a negative regulator of point mutation introduction9; however, MMR does not process loops or bulges larger than 16 nucleotides, which would be utilized to install large insertions and deletions by prime editing.10,11,12 Therefore, the mechanism(s) by which the cell processes prime edits larger than 16-nt is unexplored. While traditional prime editing often becomes hindered by longer edits, “paired” prime editing techniques can facilitate larger genome modifications by replacing a sequence between two nick sites. 8,13 More thorough understanding of the DNA repair pathways will improve both traditional and paired prime editing. Aim 1 of this proposed project will develop a “dead” fluorescent reporter that is corrected by a large genomic modification by either traditional or paired prime editing. The reporter will be utilized in Aim 2, in which a high-throughput Cas12 knockout screen will be used to identify the repair pathways involved in processing large edits by traditional and paired prime editing. DNA damage repair genes will be knocked out, and K562 cell lines constitutively expressing the developed reporter and prime editor system will be separated by editing efficiency via fluorescent activated cell sorting (FACS), high-throughput sequencing (HTS), and large-scale bioinformatics data processing. In Aim 3, the results from the genomic screen will be validated through testing at multiple endogenous sites and in various cell types to ensure reproducibility and rigor in identifying hit genes. With an increased understanding of traditional and paired prime editing, the resulting gene hits will be incorporated into novel prime editor systems to increase editing efficiency for longer edits, which can fill gaps on treating all genetic disease. Additional knowledge of what repair pathways process large edits will also add to the DNA damage repair field, enriching understanding on structure and biology of genomes. Ultimately, the proposed training plan is set to advance the PI’s technical foundation of genomic, high-throughput screening methods, as well as provide steps to develop professional skills, leadership, and scientific independence for a future in academic research. It will be supported by the sponsor Dr. Alexis Komor and UCSD’s Chemistry and Biochemistry department, in consortium with the many UCSD and Sanford core research facilities that will provide the necessary FACS, HTS, and supercomputing instruments.

Up to $43K

2029-04-30

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High-throughput tools to track the fate of prematurely terminated transcripts in cancer cells

NCI - National Cancer Institute

Post-transcriptional gene regulation plays a critical but understudied role in cancer development. The genetic basis of cancer has been extensively characterized; yet, alterations in RNA processing mechanisms, such as alternative polyadenylation (APA) events, can also drive oncogenesis independent of DNA mutations. In chronic lymphocytic leukemia, APA inactivates tumor suppressor genes by generating intronically terminated transcripts that produce truncated proteins lacking tumor-suppressive domains. Similar APA-driven oncogenic mechanisms have been observed in glioblastoma and lung adenocarcinoma. APA events in 3’ UTRs are prevalent in many cancer types, leading to changes in stability, localization, and translation of these transcripts, with widespread ramifications including increased cell proliferation, angiogenesis, and metastasis. Despite the recognition that APA events play a significant role in cancer, technological limitations have hampered our ability to track the dynamic fate and functional impact of alternatively processed transcripts across the cell in different cancer types. Current methods that identify APA events cannot simultaneously monitor the nuclear export, translation efficiency, or degradation patterns of APA transcripts to understand their movement and fate in the cell. A better understanding of the dynamics of APA transcripts, and how they differ in cancer cells versus healthy cells, will help us to define critical regulatory nodes for these transcripts, and uncover cellular processes that promote the usage of oncogenic APA transcripts across different cancer types. To quantify RNA flow rates across subcellular compartments, from synthesis to export, translation, and degradation, we developed subcellular TimeLapse-seq (TL-seq). While this technology establishes a foundation for investigating RNA dynamics, it has not yet been engineered to track the fate of transcripts that are alternatively polyadenylated (APA transcripts). Here, we propose to integrate subcellular TL-seq with 3'-end sequencing to create powerful new tools for comprehensively studying the flow and fate of alternatively polyadenylated transcripts in cancer cells. Aim 1: To map the fate of APA transcripts, we will develop 3prime-TLseq, a novel method that combines subcellular TimeLapse-seq with 3'-end sequencing to quantify APA transcript dynamics. To demonstrate the utility of our approach, we will apply the developed protocol to investigate how JTE-607 – a small molecule that interferes with cleavage and polyadenylation machinery and shows promising activity against various cancer types– affects the dynamics of APA transcripts. Aim 2: To determine whether and how well APA transcripts are translated, we will develop 3primeTL-polysome profiling, which combines TimeLapse-seq and 3'-end sequencing with polysome profiling to quantitatively measure both the rate of RNA loading onto polysomes and the half-life of associated RNAs. By overcoming the limitations of current methods, which can identify global APA events but cannot track their dynamic flow and fate through subcellular compartments, we will enable researchers to study previously inaccessible aspects of RNA regulation in cancer, and potentially identify novel therapeutic vulnerabilities.

Up to $237K

2029-02-28

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Highland Park Playscape-Construction Drawings

Friends of Highland Park Elementary

The Friends of Highland Park Elementary will hire a designer to create construction drawings based on our Conceptual Site Plan which was developed with a Small and Simple award. We will continue outreach efforts and work to ensure that the spaces created are inviting to the many cultures present in our community.

Up to $25K

artsinfrastructure

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Hijacking the neonatal Fc receptor: the novel biology of arterivirus entry, transmission, and persistence

NIAID - National Institute of Allergy and Infectious Diseases

PROJECT SUMMARY/ABSTRACT Understanding novel mechanisms by which animal viruses enter cells, evade immunity, and cause disease has scientific and public health importance. Arteriviruses are an understudied family of RNA viruses (related to coronaviruses) that infect a wide variety of mammals. The host and viral factors that determine arterivirus disease, persistence, and cross-species transmission remain unknown and unpredictable. This lack of understanding has implications for predicting/thwarting arterivirus emergence in humans, as some arteriviruses have been shown to infect human cells. Macrophages are exclusively infected by most arteriviruses, but the subpopulation(s) of macrophages that support arterivirus replication remain poorly defined. The process by which arteriviruses enter cells is highly novel and also poorly understood. Each virion displays an unusually large set of surface glycoproteins (7-11 depending on the virus) that are unlike any known viral fusion machinery. The macrophage-specific molecule CD163 is a required arterivirus receptor, yet CD163 by itself is insufficient to mediate arterivirus entry. We recently identified the neonatal Fc receptor (FcRn) as an important entry factor that arteriviruses use together with CD163 to gain entry into cells. Aim 1 of this project builds upon this discovery to define the molecular details of the arterivirus:FcRn interaction. In Aim 1a, we will map the site(s) on FcRn that are critical for arterivirus engagement by creating chimeric FcRn molecules that incorporate features of arterivirus-permissive and -resistant FcRn orthologs, with the goal of defining the domains, motifs, and residues involved in arterivirus/FcRn interactions. In Aim 1b, we will generate chimeric arteriviruses that contain combinations of glycoproteins from different arteriviruses, seeking to define the viral glycoproteins, domains, motifs, and residues involved in FcRn engagement. In Aim 1c, we will continue to develop our understanding of the host factors required for arterivirus entry by performing screens to identify additional host factors that are functionally redundant with FcRn for the viral entry process. In Aim 2, we will use the murine arterivirus (lactate dehydrogenase-elevating virus, LDV), which causes life-long viremia in adult mice, to understand several aspects of arterivirus infection in vivo. In Aim 2a, we will use a nanoluciferase-expressing LDV to perform body-wide imaging and identify the tissues that support LDV infection over time. In Aim 2b, we will hone in key tissues and identify the macrophage populations within these tissues that support LDV infection and determine how persistent arterivirus infection impacts recovery of target cell populations. Finally, in Aim 2c we will use FcRn-knockout mice to determine whether arteriviruses hijack FcRn’s physiologic role in placental biology, potentially explaining the high efficiency with which arteriviruses transmit vertically. This project will provide insights into novel mechanisms of viral entry, macrophage infection and dysfunction, viral persistence, and vertical transmission through the study of the neglected “pre-emergent” family of mammalian viruses, the arteriviruses.

Up to $615K

2031-03-31

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HiJAKing the System: Investigating the Impact Mechanisms of Low Variant Allele Frequency JAK2V617F Clone on the Pathobiology of Myeloproliferative Neoplasms

NCI - National Cancer Institute

PROJECT SUMMARY/ABSTRACT JAK2V617F (JAK2V/F) is one of the Clonal Hematopoiesis if Indeterminate Potential (CHIP)-associated mutations and a major driver of myeloproliferative neoplasms (MPN). JAK2V/F is present in diverse MPN phenotypes ranging from asymptomatic low-variant allele frequency (VAF) CHIP, erythroid-specific expansion in polycythemia vera (PV), platelet overproduction in essential thrombocythemia (ET), bone marrow (BM) scarring in primary myelofibrosis (PMF) or MPN transformation into acute myeloid leukemia (AML). Highly specific JAK inhibitors fail to provide curative outcomes of MPNs, therefore searching for alternative treatment strategies is critically needed. We recently created a traceable murine JAK2V/F MPN model in which the disease is induced in the unconditioned recipients (CD45.1) of BM transplantation (BMT) with a low-VAF JAK2V/F donor clone (CD45.2). Whole BMT was performed via a single injection of the JAK2V/F donor cells into the recipients, which resulted in development of a PV-like phenotype with an average chimerism of 2.7% in the recipient peripheral blood. Post-BMT, RNA-seq analysis of the sorted CD45.2+ BM cells showed an increase in transcripts specific to early erythroblast and myeloid cells, and downregulation of those specific to lymphoid (B, T, NK) cells. Unexpectedly, transcripts specific to myeloid cells and erythroblasts also increased in the sorted CD45.1+ BM cells not bearing JAK2V/F, suggesting the BMT recipients had a skewed hematopoiesis towards a myeloproliferative phenotype. Furthermore, RNA-seq analysis of the recipient BM stroma found a loss of osteo- mesenchymal transcripts, which was corroborated by a reduction of trabecular bone observed in µCT imaging. Based on these data, we hypothesize that JAK2V/F clone, even presented at low frequencies, profoundly impacts the host hematopoietic system as well as the BM stroma, thus contributing to the MPN and multiorgan dysfunction. To test this hypothesis we will use our developed BMT strategy and cell-type specific murine models- enabled single cell RNA sequencing coupled with secretome (cytokine/chemokine, proteome and metabolome) profiling to delineate the cellular and acellular factors that mediate pathogenic effects of the JAK2V/F clone on non-mutated hematopoietic cells (Aim 1). Using our established model of BMT w/o conditioning and DARLIN lineage tracing mouse line in serial BMT approach we will determine the MPN disease-inducing potential of JAK2V617F+ clone-exposed (primed) unmutated hematopoietic cells (Aim 2). Using mesenchymal progenitor (LepR+)-specific reporter mice as unconditioned BMT recipients, we will examine the impact of JAK2V/F clone on the differentiation and function of BM mesenchymal stromal cells (Aim 3). Our data indicate that JAK2V/F CHIP clone at low frequency induces profound and irreversible alterations in its microenvironment, which then prime pathogenic phenotype of the unmutated bystander cells. The outcome of this study will generate foundational information to explain why the JAK2V/F-targeted therapies are ineffective and inform new directions in MPN therapies.

Up to $689K

2031-04-30

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Hilarity for Charity — Alzheimer's Care Grants

Alzheimers carein-home carefamily support

Hilarity for Charity (HFC)

Grants for programs providing in-home care, adult day programs, and support groups for families affected by Alzheimer's.

$10K – $50K

2026-07-31

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Hillman City P-Patch Accessible Paths and Planning

Hillman City P-Patch

The Hillman City P-Patch garden community plans to reclaim the accessible gravel paths and develop an accessible gardening area at the site. The project also includes an update to their 2010 visioning plan and the initiation of a monthly serieis of events designed to engage the gardeners, neighbors and partner organizations.

Up to $15K

general

Free to search & build · $99 one-time to unlock the application pack · No subscription

Hillsborough County Arts — Project Grant

artsculturecommunity arts

Hillsborough County Arts

Project and operating grants for arts organizations and individual artists from Hillsborough County Arts. Supports visual, performing, literary, and media arts.

$2K – $50K

2026-12-31

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Hillsborough County Economic Development Grants

Hillsborough County Economic Development Department

Economic development incentives and grants supporting business growth, job creation, and workforce development in the Tampa/Hillsborough County area.

$10K – $200K

Business Developmentworkforce

Free to search & build · $99 one-time to unlock the application pack · No subscription

Hindu American Foundation Community Grants

Hindu American Foundation

Supports Hindu American advocacy, education, and community development. Promotes pluralism, religious liberty, and Hindu cultural heritage.

$5K – $50K