Grants

Tell Every Amazing Lady About Ovarian Cancer Foundation

Local

City of Richmond

Axial to Terminal

City of Oakland

Support for Artistic and Educational Programs. New works will include Contingencies by L.A. based choreographer Victoria Marks with an original music composition by Beth Custer, plus three works by Bay Area based choreographers Amy Seiwart, Alex Ketley and our own dancer Sebastian Grubb. 

NYC Department of Cultural Affairs

Ayazamana Cultural Center

Storefront for Art and Architecture

Azin Valy - Valy: PALETTE HOUSE, TRANSITIONAL REFUGEE HOUSING

City of Richmond

Aziz Bourema

AZTEC SUPPLY CORPORATION

Defense and Law Enforcement and Security and Safety Equipment and Supplies

Command Souring, Inc.

Defense and Law Enforcement and Security and Safety Equipment and Supplies

Sharon

Babe Ruth Field Fence Replacement

Cambridge Performance Project, Inc.

Back Pocket Dancers

Pro Arte Chamber Orchestra

Back to Classical

Cambridge Educational Access

Back to the 'Bridge: CRLS Alumni Artists in Residence

Artspace Committee

The Artspace Committee will host a free public event featuring games, food, art, and karaoke based on a 1980's cultural theme. It will be held at Artspace Hiawatha Lofts located at 843 Hiawatha Pl S. The event will take place on May 26, 2018 from 5 pm - 10 pm.

City of Cannon Beach

Backbone Resiliency Project Phase 1

City of Cannon Beach

Backbone Resiliency Project Phase 1

NIAID - National Institute of Allergy and Infectious Diseases

Project Summary The host lipid environment is a well-described barrier to bacterial infection at several sites including the skin, lung, and upper airways. In addition, both bacterial and host lipids serve as signals that promote inflammation. Bacterial lipids activate Toll-like receptors which initiates cytokine production, immune cell recruitment, and induction of oxidative burst to control infection. Several pathogenic bacteria evade these lipid-mediated defenses by secreting lipolytic enzymes (lipases). Bacterial lipases can cause host membrane dissolution, facilitate escape from cellular compartments, disrupt physical barriers, detoxify antimicrobial lipids, and release nutrient fatty acids. Despite these established contributions of lipases to infection there exist critical gaps in knowledge on how lipases drive host immunity and physiology. This is especially true for Staphylococcus aureus which produces three lipases that have long been presumed to promote virulence, despite scant mechanistic studies. Recent work from our lab started to fill this gap by providing evidence for a direct role for lipases in immune evasion and nutrient acquisition during infection. We made the unexpected observation that the S. aureus secreted lipase, Geh, inhibited activation of innate immune cells in culture. Further, Geh blunted pro-inflammatory cytokine production during infection and was responsible for bacterial persistence in some tissues but not others, highlighting tissue-specific contributions of Geh to survival. The blunted cytokine response was not due to direct functions of Geh on mammalian cells, but rather a result of inactivation of S. aureus lipoproteins, a major pathogen-associated molecular pattern (PAMP) of Gram-positive bacteria, via ester hydrolysis. While our studies indicate S. aureus uses lipases to inactivate lipoprotein PAMPs containing saturated straight and branched chain fatty acids that it synthesizes de novo, it can also acquire host unsaturated fatty acids in a lipase-dependent manner and use them for lipoprotein biogenesis. We found that S. aureus uptake of host fatty acids disrupted innate immune responses and infection dynamics in tissues involved in lipid metabolism via attachment of host fatty acids to bacterial lipoproteins. This deleterious response correlated well with Toll-like receptor 2 signaling. Finally, infections of the skin uncovered a role for Geh that appeared to be independent of Toll-like receptor 2 and instead was linked to liberation of host fatty acid esters that promoted fitness. Altogether, our data support the hypothesis that S. aureus lipases modify both bacterial and host lipids to calibrate innate immunity and promote a fitness advantage that allows adaptation to a wide range of tissues during infection. Aim 1 will determine the cellular and molecular basis for Geh-mediated infection persistence. Aim 2 will test the roles of the three lipases secreted by S. aureus in virulence, lipid utilization, and immune defense. Aim 3 will determine how lipases disrupt immunity and allow S. aureus to adapt to increased circulating lipids in the host.

NIGMS - National Institute of General Medical Sciences

PROJECT SUMMARY Bacteria have successfully colonized almost every niche on this planet, and their success is driven in large part by their ability to adapt to and survive environmental challenges. One example is excessive protons (or acidity, low pH), which can damage lipids, nucleic acids, and proteins. Bacteria that colonize the human body contend with many different pH gradients, especially in the gastrointestinal system. One way that bacteria survive this stress is by using amino acid catabolism to de-acidify their environment. We discovered that the bacterial second messenger c-di-GMP regulates both amino acid metabolism and acid resistance in the gastrointestinal bacteria E. coli and Shigella. One of these amino acids is isoleucine, and we have found that E. coli, but not Shigella, catabolizes isoleucine to resist acid stress. Bacterial isoleucine-mediated acid resistance has not been previously reported. In this application, we will investigate how isoleucine contributes to acid resistance, how c- di-GMP regulates acid resistance in these two organisms, and how these pathways are impacted by the evolutionary process of pathoadaptation. Methodologies proposed here are accessible for undergraduate researchers, who will complete the experiments outlined in this proposal. Because many bacteria use amino acid metabolism to resist acid stress, and because c-di-GMP signaling is so widely conserved, findings from this study will be widely generalizable for other gastrointestinal bacteria. Understanding how gastrointestinal bacteria adapt to pH stress is critical for many aspects of human health, including protecting our food supply chain, promoting symbiotic bacteria, and controlling human pathogens.

Ray O'Herron Company, Inc.

Badges

City of Richmond

MAX CAB MEDALLION 885

Wild Rivers Land Trust

Bagley Creek Mill Site environmental assessment

Wild Rivers Land Trust

Bagley Creek/Frmr Western States Plywood Mill Site

The Amigos School

Bailamos Juntos

North Haven

Bailey Road Baseball Field Backstop and Fencing Improvements

NYC Department of Cultural Affairs

Bailey's Cafe