Development of molecular tools and mouse models to study neglected Sindbis virus genotypes and their epidemic potential
NIAID - National Institute of Allergy and Infectious Diseases
About This Grant
Title: Development of molecular tools and mouse models to study neglected Sindbis virus genotypes and their epidemic potential PROJECT SUMMARY Sindbis virus (SINV) is a mosquito-borne alphavirus with a single-stranded positive-sense RNA genome. SINV is transmitted between mosquitoes and birds which serve as reservoir hosts for the virus but can cause spillover infections in other vertebrate hosts including humans. SINV can be classified into distinct genotypes (G1-G6) which aligns with their zoogeographical distribution. SINV causes frequent epidemic outbreaks of polyarthritis disease in Northern Europe and has also been associated with outbreaks in Africa. Epidemics in both these regions are caused by G1 viruses. SINV is widely distributed geographically but is not associated with epidemics elsewhere in the world. Despite being prevalent in mosquito populations in Australia, the SINV strains in this region (G2/3 and G6) have never been associated with epidemics of polyarthritis disease here, though isolated human cases have been reported, and serological data shows the occurrence of human infections. The Australian genotypes of SINV (G2/3, G6) are significantly divergent from the G1 viruses which cause frequent epidemics, suggesting that genetic differences between these genotypes may explain why G1 viruses have a propensity to cause epidemics but other genotypes do not. Most of the genetic tools available to study SINV are derived from G1 viruses, and there is only a single non-G1 cDNA clone (for the G4 virus XJ-160). There currently exists no molecular clones for studying other genotypes of SINV, including the divergent Australian G2 and G6 viruses. In this R03 we propose to construct cDNA clones for G2 and G6 SINV and characterize the in vitro replication properties of these clones and compare pathogenesis profiles of virus derived from these clones versus characterized G1 strains in a small animal model. We have established a subcutaneous model of SINV infection and shown that replicating virus can be detected in the skin and joints of adult mice, which reflects the pathogenesis observed in humans. Thus, this serves as a good model for examining the genetic determinants that underlie the epidemic potential of neglected SINV genotypes. These tools will be available to the research community and thus aid in further investigations of other key aspects of arbovirus transmission and pathogenesis including virus-mosquito interactions, species-specific determinants of transmission, and viral immunity.
Grant Summary
Development of molecular tools and mouse models to study neglected Sindbis virus genotypes and their epidemic potential is a NIAID - National Institute of Allergy and Infectious Diseases grant providing up to $78K for university, nonprofit, healthcare org. Applications are due 2028-03-31 (open). Check eligibility and apply with FindGrants.
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Up to $78K
2028-03-31
- 1Confirm your organization is eligible for Development of molecular tools and mouse models to study neglected Sindbis virus genotypes and their epidemic potential from NIAID - National Institute of Allergy and Infectious Diseases, checking organization type, location, and any population or project requirements.
- 2Gather the required documents and information, including your organization details, project plan, and budget figures.
- 3Draft your application narrative and budget addressing the funder's priorities and review criteria. FindGrants can draft each section for you to review and edit.
- 4Review every section against the requirements checklist, then export a submission-ready application pack and submit it to NIAID - National Institute of Allergy and Infectious Diseases before the deadline.
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Development of molecular tools and mouse models to study neglected Sindbis virus genotypes and their epidemic potential: Frequently Asked Questions
Who is eligible for the Development of molecular tools and mouse models to study neglected Sindbis virus genotypes and their epidemic potential?
Development of molecular tools and mouse models to study neglected Sindbis virus genotypes and their epidemic potential is offered by NIAID - National Institute of Allergy and Infectious Diseases and is generally open to university, nonprofit, healthcare org. It is open to organizations nationwide unless the funder specifies otherwise. Review the specific eligibility terms before applying, since funders set their own requirements around organization type, location, and the population or project being served.
How much funding does the Development of molecular tools and mouse models to study neglected Sindbis virus genotypes and their epidemic potential provide?
Development of molecular tools and mouse models to study neglected Sindbis virus genotypes and their epidemic potential provides up to $78K per award from NIAID - National Institute of Allergy and Infectious Diseases. Actual award sizes depend on the scope of your project, available program funds, and the number of applicants, so build a budget that reflects realistic, allowable costs rather than the maximum figure.
When is the Development of molecular tools and mouse models to study neglected Sindbis virus genotypes and their epidemic potential deadline?
Applications for Development of molecular tools and mouse models to study neglected Sindbis virus genotypes and their epidemic potential are due 2028-03-31 (open). Because deadlines can change, verify the date with the funder, NIAID - National Institute of Allergy and Infectious Diseases, and give yourself enough time to prepare a complete, competitive application before the close date.
How do you apply for the Development of molecular tools and mouse models to study neglected Sindbis virus genotypes and their epidemic potential?
To apply for Development of molecular tools and mouse models to study neglected Sindbis virus genotypes and their epidemic potential, confirm your eligibility, gather the required documents, and prepare a narrative and budget that address the funder's priorities. FindGrants guides you step by step and can draft each section, then exports a submission-ready application pack for this grant from NIAID - National Institute of Allergy and Infectious Diseases.