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View full policySwine Somatic Cell Gene Editing Testing Center (Targeted Challenge Testing Center Independent Validation)
About This Grant
Project Summary After further deliberation from the ‘Targeted Challenge’ Working Group at NIH. It was determined that additional animals are required for the Programmable Delivery System effort, to be generated by three breeding cohorts of sows. Therefore, this administrative supplement reflects the same project summary, narrative, and research strategy as 3U42OD035738-03W1. The objective of this supplement project is to perform a side-by-side assessment of various gene editing (GE) formulations to identify those most effective as reagents for tissue editing. The project involves the delivery of CRISPR/Cas9 gene editing ribonucleoprotein complexes intravenously to pigs. Fluorescent reporter pigs will be used to detect editing activity and cell transduction efficiency. Successful targeting in cells and tissues by the formulations will be demonstrated by the induced expression of a red fluorescent protein (tdTomato). The effort for this project is structured into three parts: 1) In Vitro evaluations of test reagents, 2) In Vivo Toxicity Pilot delivery, and 3) final In Vivo Delivery to complete the animal studies. Importantly, all the reagents will utilize the same guide RNA from a common source that will target the same sites for editing. After each delivery, the pigs will be monitored daily, and blood will be drawn frequently. Inflammatory cytokines will be measured as well as serum chemistry levels and blood CBC and differentials, using the standard toxicology package provided by the University of Missouri Veterinary Medicine Diagnostic Laboratory (VMDL). At the close of the study (4 weeks ±2 days post-delivery), animals will be euthanized, and gross necropsies will be performed by the Testing Center staff. Tissues will be harvested, fixed, embedded, and sectioned for histopathology evaluation and imaging of tdTomato (or other immunostaining, based on project needs). For ‘Programmable Delivery’, three project-defined target tissues, along with the Liver and Thoracic Dorsal Root Ganglion will be evaluated. Ultimately, these evaluations will include H&E, cell-specific markers in serial sections to determine which cell type(s) were transduced, and high-resolution fluorescent imaging of the most targeted tissues. A detailed summary of the imaging assessments, blood panels, and circulating inflammatory markers will be provided to the targeted challenge board for their rankings. At the end of the study, the results and tissues will be provided to the submitting investigator teams.
Grant Summary
Swine Somatic Cell Gene Editing Testing Center (Targeted Challenge Testing Center Independent Validation) is a OD - NIH Office of the Director grant providing up to $103K for university, nonprofit, healthcare org. Applications are due 2026-08-31 (open). Check eligibility and apply with FindGrants.
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Focus Areas
Eligibility
How to Apply
Up to $103K
2026-08-31
- 1Confirm your organization is eligible for Swine Somatic Cell Gene Editing Testing Center (Targeted Challenge Testing Center Independent Validation) from OD - NIH Office of the Director, checking organization type, location, and any population or project requirements.
- 2Gather the required documents and information, including your organization details, project plan, and budget figures.
- 3Draft your application narrative and budget addressing the funder's priorities and review criteria. FindGrants can draft each section for you to review and edit.
- 4Review every section against the requirements checklist, then export a submission-ready application pack and submit it to OD - NIH Office of the Director before the deadline.
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Swine Somatic Cell Gene Editing Testing Center (Targeted Challenge Testing Center Independent Validation): Frequently Asked Questions
Who is eligible for the Swine Somatic Cell Gene Editing Testing Center (Targeted Challenge Testing Center Independent Validation)?
Swine Somatic Cell Gene Editing Testing Center (Targeted Challenge Testing Center Independent Validation) is offered by OD - NIH Office of the Director and is generally open to university, nonprofit, healthcare org. It is open to organizations nationwide unless the funder specifies otherwise. Review the specific eligibility terms before applying, since funders set their own requirements around organization type, location, and the population or project being served.
How much funding does the Swine Somatic Cell Gene Editing Testing Center (Targeted Challenge Testing Center Independent Validation) provide?
Swine Somatic Cell Gene Editing Testing Center (Targeted Challenge Testing Center Independent Validation) provides up to $103K per award from OD - NIH Office of the Director. Actual award sizes depend on the scope of your project, available program funds, and the number of applicants, so build a budget that reflects realistic, allowable costs rather than the maximum figure.
When is the Swine Somatic Cell Gene Editing Testing Center (Targeted Challenge Testing Center Independent Validation) deadline?
Applications for Swine Somatic Cell Gene Editing Testing Center (Targeted Challenge Testing Center Independent Validation) are due 2026-08-31 (open). Because deadlines can change, verify the date with the funder, OD - NIH Office of the Director, and give yourself enough time to prepare a complete, competitive application before the close date.
How do you apply for the Swine Somatic Cell Gene Editing Testing Center (Targeted Challenge Testing Center Independent Validation)?
To apply for Swine Somatic Cell Gene Editing Testing Center (Targeted Challenge Testing Center Independent Validation), confirm your eligibility, gather the required documents, and prepare a narrative and budget that address the funder's priorities. FindGrants guides you step by step and can draft each section, then exports a submission-ready application pack for this grant from OD - NIH Office of the Director.