Asymmetric CRISPR Approach for Nucleic Acid Quantification
NIBIB - National Institute of Biomedical Imaging and Bioengineering
About This Grant
Abstract The quantitative detection of nucleic acids plays an increasingly important role in the early detection and screening of cancers. Although the real-time quantitative PCR method has become the gold standard for nucleic acid quantification, its reliance on expensive equipment and trained personnel limits its use in resource-limited settings. Recently, CRISPR technology has emerged as a simple and powerful tool for highly sensitive and specific nucleic acid detection when combined with nucleic acid pre-amplification. However, due to the incorporation of the pre-amplification step, CRISPR-based detection methods require multiple operations and lack the quantitative detection capabilities needed for nucleic acid targets. Therefore, developing a simple and sensitive CRISPR approach for the quantitative detection of nucleic acid biomarkers (e.g., microRNAs) remains a challenge. Recent studies have demonstrated that exosomal microRNAs (exo-miRNAs) are promising liquid biopsy biomarkers for detecting cancer progression and assessing therapy efficacy with high sensitivity and specificity. However, current methods for exo-miRNA detection often require expensive equipment and specialized expertise, hindering their widespread clinical application. Here, we propose to study an asymmetric CRISPR approach for the quantitative detection of nucleic acids (e.g., exo-miRNAs). Furthermore, we will integrate the asymmetric CRISPR assay into a microfluidic chip to develop an asymmetric CRISPR diagnostic platform for exo-miRNA profiling in clinical blood samples. As an example application, we will adapt our asymmetric CRISPR diagnostic platform for the non-invasive early detection of breast cancer—the most commonly diagnosed cancer in the world—and rigorously validate its clinical application. Ultimately, the success of our proposed project will enable clinical translation, facilitating the development of a simple, sensitive, nucleic acid-based molecular detection method for early cancer detection and personalized medicine.
Grant Summary
Asymmetric CRISPR Approach for Nucleic Acid Quantification is a NIBIB - National Institute of Biomedical Imaging and Bioengineering grant providing up to $644K for university, nonprofit, healthcare org. Applications are due 2030-01-31 (open). Check eligibility and apply with FindGrants.
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How to Apply
Up to $644K
2030-01-31
- 1Confirm your organization is eligible for Asymmetric CRISPR Approach for Nucleic Acid Quantification from NIBIB - National Institute of Biomedical Imaging and Bioengineering, checking organization type, location, and any population or project requirements.
- 2Gather the required documents and information, including your organization details, project plan, and budget figures.
- 3Draft your application narrative and budget addressing the funder's priorities and review criteria. FindGrants can draft each section for you to review and edit.
- 4Review every section against the requirements checklist, then export a submission-ready application pack and submit it to NIBIB - National Institute of Biomedical Imaging and Bioengineering before the deadline.
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Asymmetric CRISPR Approach for Nucleic Acid Quantification: Frequently Asked Questions
Who is eligible for the Asymmetric CRISPR Approach for Nucleic Acid Quantification?
Asymmetric CRISPR Approach for Nucleic Acid Quantification is offered by NIBIB - National Institute of Biomedical Imaging and Bioengineering and is generally open to university, nonprofit, healthcare org. It is open to organizations nationwide unless the funder specifies otherwise. Review the specific eligibility terms before applying, since funders set their own requirements around organization type, location, and the population or project being served.
How much funding does the Asymmetric CRISPR Approach for Nucleic Acid Quantification provide?
Asymmetric CRISPR Approach for Nucleic Acid Quantification provides up to $644K per award from NIBIB - National Institute of Biomedical Imaging and Bioengineering. Actual award sizes depend on the scope of your project, available program funds, and the number of applicants, so build a budget that reflects realistic, allowable costs rather than the maximum figure.
When is the Asymmetric CRISPR Approach for Nucleic Acid Quantification deadline?
Applications for Asymmetric CRISPR Approach for Nucleic Acid Quantification are due 2030-01-31 (open). Because deadlines can change, verify the date with the funder, NIBIB - National Institute of Biomedical Imaging and Bioengineering, and give yourself enough time to prepare a complete, competitive application before the close date.
How do you apply for the Asymmetric CRISPR Approach for Nucleic Acid Quantification?
To apply for Asymmetric CRISPR Approach for Nucleic Acid Quantification, confirm your eligibility, gather the required documents, and prepare a narrative and budget that address the funder's priorities. FindGrants guides you step by step and can draft each section, then exports a submission-ready application pack for this grant from NIBIB - National Institute of Biomedical Imaging and Bioengineering.