Acquisition of a 300 keV CryoEM/ET Instrument
About This Grant
We propose to purchase a JEOL CryoARM 300II cryo-transmission electron microscope (TEM), which will be housed in the CryoEM/ET core at Baylor College of Medicine. This instrument is a replacement for a 20 year old JEOL 3200 going off contract, with an end-of-life direct detector. Critically, the retiring instrument is incompatible with the specimens produced by our ThermoFisher Aquilos 2 Cryo-FIB/SEM. Our only compatible microscope is a ThermoFisher Glacios which operates at 200 keV and lacks an energy filter, severely degrading the quality of the tomograms we can acquire. Our Glacios has also proven to achieve only ~2.5 Å resolution, which is adequate for some single particle projects and for screening, but is not suitable for final collection of large data sets. Our core has served over 40 different research groups in the last 3 years performing a wide range of biological research including various cancers, viral diseases, the function of neurons and structures of wide ranging biomolecules. The proposed instrument is a second generation CryoARM 300, which corrects all of the minor shortcomings of the original instrument, making it an extremely attractive option at half the price of a ThermoFisher Krios. In addition to high quality CryoET data, is able to collect CryoEM single particle data for studying the structure and dynamics of large macromolecules to near-atomic resolution, with high throughput. There is only one other instrument in the Texas Medical Center, capable of performing some, but not all, of the imaging we will perform on this new instrument. That Krios is being used at very close to capacity (92% in a recent year) and it is now 7 years old. It was down several months last year, and is suffering from a sporadic detector failure requiring a ~$1.5M replacement. The proposed CryoARM has demonstrated 1.2 Å resolution reconstructions, loads clipped grids making it compatible with ThermoFisher instruments, and has all of the other features required by our users. Its sole disadvantage is that it is not as thoroughly automated, requiring a more skilled user to achieve equivalent throughput. We believe our in-house expertise with JEOL instruments will allow us to achieve excellent productivity with this instrument in addition to providing the highest quality data and reducing weeks-long delays in our current workflows to hours.
Grant Summary
Acquisition of a 300 keV CryoEM/ET Instrument is a NIGMS - National Institute of General Medical Sciences grant providing up to $2M for university, nonprofit, healthcare org. Applications are due 2027-06-30 (open). Check eligibility and apply with FindGrants.
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How to Apply
Up to $2M
2027-06-30
- 1Confirm your organization is eligible for Acquisition of a 300 keV CryoEM/ET Instrument from NIGMS - National Institute of General Medical Sciences, checking organization type, location, and any population or project requirements.
- 2Gather the required documents and information, including your organization details, project plan, and budget figures.
- 3Draft your application narrative and budget addressing the funder's priorities and review criteria. FindGrants can draft each section for you to review and edit.
- 4Review every section against the requirements checklist, then export a submission-ready application pack and submit it to NIGMS - National Institute of General Medical Sciences before the deadline.
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Acquisition of a 300 keV CryoEM/ET Instrument: Frequently Asked Questions
Who is eligible for the Acquisition of a 300 keV CryoEM/ET Instrument?
Acquisition of a 300 keV CryoEM/ET Instrument is offered by NIGMS - National Institute of General Medical Sciences and is generally open to university, nonprofit, healthcare org. It is open to organizations nationwide unless the funder specifies otherwise. Review the specific eligibility terms before applying, since funders set their own requirements around organization type, location, and the population or project being served.
How much funding does the Acquisition of a 300 keV CryoEM/ET Instrument provide?
Acquisition of a 300 keV CryoEM/ET Instrument provides up to $2M per award from NIGMS - National Institute of General Medical Sciences. Actual award sizes depend on the scope of your project, available program funds, and the number of applicants, so build a budget that reflects realistic, allowable costs rather than the maximum figure.
When is the Acquisition of a 300 keV CryoEM/ET Instrument deadline?
Applications for Acquisition of a 300 keV CryoEM/ET Instrument are due 2027-06-30 (open). Because deadlines can change, verify the date with the funder, NIGMS - National Institute of General Medical Sciences, and give yourself enough time to prepare a complete, competitive application before the close date.
How do you apply for the Acquisition of a 300 keV CryoEM/ET Instrument?
To apply for Acquisition of a 300 keV CryoEM/ET Instrument, confirm your eligibility, gather the required documents, and prepare a narrative and budget that address the funder's priorities. FindGrants guides you step by step and can draft each section, then exports a submission-ready application pack for this grant from NIGMS - National Institute of General Medical Sciences.