Developing the Next Generation of Genome-Editing Tools for Large Cargo DNA Integration
About This Grant
Project Summary/Abstract Our laboratory investigates the molecular mechanisms and engineering potential of CRISPR-associated transposons (CASTs)—a novel class of RNA-guided integrases capable of inserting DNA into specific genomic loci without requiring double-strand breaks or homologous recombination. These systems represent a fundamentally new paradigm in genome editing, with implications for both basic science and translational biotechnology. Over the past several years, we have uncovered key mechanistic features of CAST function, including the allosteric regulation of transposase activity and the involvement of host-encoded factors that stabilize the integration complex. Looking ahead, our vision is to transform CASTs into a versatile and efficient platform for programmable genome integration, especially in challenging contexts such as human cells. Over the next five years, we will build on our foundational work by combining structural biology, high-throughput screening, and machine-learning-based protein design to re-engineer CAST components for enhanced activity, specificity, and portability across species. We aim to understand and exploit the modularity of these systems to expand their targeting range, simplify their composition, and reduce reliance on host factors. Through these efforts, our long-term goal is to create a next-generation genome editing toolkit that enables safe, precise, and programmable gene insertion for therapeutic and synthetic biology applications.
Grant Summary
Developing the Next Generation of Genome-Editing Tools for Large Cargo DNA Integration is a NIGMS - National Institute of General Medical Sciences grant providing up to $315K for university, nonprofit, healthcare org. Applications are due 2031-06-30 (open). Check eligibility and apply with FindGrants.
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Focus Areas
Eligibility
How to Apply
Up to $315K
2031-06-30
- 1Confirm your organization is eligible for Developing the Next Generation of Genome-Editing Tools for Large Cargo DNA Integration from NIGMS - National Institute of General Medical Sciences, checking organization type, location, and any population or project requirements.
- 2Gather the required documents and information, including your organization details, project plan, and budget figures.
- 3Draft your application narrative and budget addressing the funder's priorities and review criteria. FindGrants can draft each section for you to review and edit.
- 4Review every section against the requirements checklist, then export a submission-ready application pack and submit it to NIGMS - National Institute of General Medical Sciences before the deadline.
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Developing the Next Generation of Genome-Editing Tools for Large Cargo DNA Integration: Frequently Asked Questions
Who is eligible for the Developing the Next Generation of Genome-Editing Tools for Large Cargo DNA Integration?
Developing the Next Generation of Genome-Editing Tools for Large Cargo DNA Integration is offered by NIGMS - National Institute of General Medical Sciences and is generally open to university, nonprofit, healthcare org. It is open to organizations nationwide unless the funder specifies otherwise. Review the specific eligibility terms before applying, since funders set their own requirements around organization type, location, and the population or project being served.
How much funding does the Developing the Next Generation of Genome-Editing Tools for Large Cargo DNA Integration provide?
Developing the Next Generation of Genome-Editing Tools for Large Cargo DNA Integration provides up to $315K per award from NIGMS - National Institute of General Medical Sciences. Actual award sizes depend on the scope of your project, available program funds, and the number of applicants, so build a budget that reflects realistic, allowable costs rather than the maximum figure.
When is the Developing the Next Generation of Genome-Editing Tools for Large Cargo DNA Integration deadline?
Applications for Developing the Next Generation of Genome-Editing Tools for Large Cargo DNA Integration are due 2031-06-30 (open). Because deadlines can change, verify the date with the funder, NIGMS - National Institute of General Medical Sciences, and give yourself enough time to prepare a complete, competitive application before the close date.
How do you apply for the Developing the Next Generation of Genome-Editing Tools for Large Cargo DNA Integration?
To apply for Developing the Next Generation of Genome-Editing Tools for Large Cargo DNA Integration, confirm your eligibility, gather the required documents, and prepare a narrative and budget that address the funder's priorities. FindGrants guides you step by step and can draft each section, then exports a submission-ready application pack for this grant from NIGMS - National Institute of General Medical Sciences.