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View full policyCryopreserved Zebrafish Embryos as a Resource: Dissemination for Global Research Impact
About This Grant
ABSTRACT Zebrafish (Danio rerio) are a critical model organism in genetic and biomedical research due to their genetic similarity to humans, rapid embryonic development, and utility in studying a wide range of diseases. Researchers have over 45,000 zebrafish lines, but maintaining these genetic resources is costly, logistically challenging, and prone to genetic drift, particularly for lines with complex genetics and/or mitochondrial and maternal traits that cannot be preserved via sperm cryopreservation. Reliable zebrafish embryo cryopreservation methods would resolve these challenges, enabling long-term storage and recovery of entire (diploid and mitochondrial) genomes. Our group has developed the first successful zebrafish embryo cryopreservation protocol, combining microinjection of cryoprotectants (CPAs) and plasmonic nanoparticles with laser rewarming. This method has demonstrated survival to adulthood with normal fecundity and physiology, with recent refinements achieving near 10% survival rate to day 5 post-fertilization. To accelerate the cryopreservation and archiving of critical zebrafish stocks, we will develop, standardize, and disseminate resources to individual labs and stock centers. Significant efforts will be devoted to developing reliable methods for cryogenic storage and shipping of zebrafish embryos as well as to study if any significant mutagenic changes occur due to cryopreservation. We will be working with multiple groups within the cryobiology and zebrafish community to gather feedback on and refine our approach. With our collaboration with the Zebrafish International Resource Center (ZIRC), we will identify and prioritize the stocks that need cryopreservation to prevent their total loss in the event of a disaster or genetic drift. We will also demonstrate the efficacy of our cryopreservation protocol on a large scale with a selected group of wild-type and mitochondrial mutant lines identified by the Ekker Lab at UT Austin. Finally, we will also estimate the costs for cryopreservation in comparison to the traditional method of zebrafish maintenance to inform future efforts to protect these precious genetic resources. By reducing the financial and logistical burden of maintaining live zebrafish lines, this project will streamline genetic resource management, preserve complex genetic traits, and enhance global accessibility and collaboration in zebrafish research. This is a multi-PI project led by investigators with significant experience in zebrafish and cryobiology.
Grant Summary
Cryopreserved Zebrafish Embryos as a Resource: Dissemination for Global Research Impact is a OD - NIH Office of the Director grant providing up to $732K for university, nonprofit, healthcare org. Applications are due 2030-02-28 (open). Check eligibility and apply with FindGrants.
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Focus Areas
Eligibility
How to Apply
Up to $732K
2030-02-28
- 1Confirm your organization is eligible for Cryopreserved Zebrafish Embryos as a Resource: Dissemination for Global Research Impact from OD - NIH Office of the Director, checking organization type, location, and any population or project requirements.
- 2Gather the required documents and information, including your organization details, project plan, and budget figures.
- 3Draft your application narrative and budget addressing the funder's priorities and review criteria. FindGrants can draft each section for you to review and edit.
- 4Review every section against the requirements checklist, then export a submission-ready application pack and submit it to OD - NIH Office of the Director before the deadline.
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Cryopreserved Zebrafish Embryos as a Resource: Dissemination for Global Research Impact: Frequently Asked Questions
Who is eligible for the Cryopreserved Zebrafish Embryos as a Resource: Dissemination for Global Research Impact?
Cryopreserved Zebrafish Embryos as a Resource: Dissemination for Global Research Impact is offered by OD - NIH Office of the Director and is generally open to university, nonprofit, healthcare org. It is open to organizations nationwide unless the funder specifies otherwise. Review the specific eligibility terms before applying, since funders set their own requirements around organization type, location, and the population or project being served.
How much funding does the Cryopreserved Zebrafish Embryos as a Resource: Dissemination for Global Research Impact provide?
Cryopreserved Zebrafish Embryos as a Resource: Dissemination for Global Research Impact provides up to $732K per award from OD - NIH Office of the Director. Actual award sizes depend on the scope of your project, available program funds, and the number of applicants, so build a budget that reflects realistic, allowable costs rather than the maximum figure.
When is the Cryopreserved Zebrafish Embryos as a Resource: Dissemination for Global Research Impact deadline?
Applications for Cryopreserved Zebrafish Embryos as a Resource: Dissemination for Global Research Impact are due 2030-02-28 (open). Because deadlines can change, verify the date with the funder, OD - NIH Office of the Director, and give yourself enough time to prepare a complete, competitive application before the close date.
How do you apply for the Cryopreserved Zebrafish Embryos as a Resource: Dissemination for Global Research Impact?
To apply for Cryopreserved Zebrafish Embryos as a Resource: Dissemination for Global Research Impact, confirm your eligibility, gather the required documents, and prepare a narrative and budget that address the funder's priorities. FindGrants guides you step by step and can draft each section, then exports a submission-ready application pack for this grant from OD - NIH Office of the Director.