Cross-species analysis of regulatory function using synthetic regulatory genomics
About This Grant
Project Abstract Understanding the evolution of regulatory DNA has been impeded both by the lack of functional genomics data available outside of a select group of organisms, as well as by the barriers to implementing genome engineering in diverse organisms for functional analysis. Given the low conservation of the genomic regulatory landscape, this presents a serious impediment to studying genome evolution and limits the value of a phylogenetic approach to understanding human genomic function. We recently developed the Big-IN genome engineering technology to rewrite large genomic loci in-place through delivery of DNA payloads upwards of 160 kb. Here we propose a new synthetic regulatory genomics approach for characterization of the regulatory function of genomic sequence across a phylogenetic tree. We will analyze the activity of a set of model loci in mouse embryonic stem cells by delivering orthologous sequences from 5 vertebrate species. We will employ two strategies to: (i) deliver larger regions (up to 160 kb) including the full gene replacing full orthologous mouse locus; and (ii) combinatorially assess pairs of shorter (~1 kb) candidate enhancer sequences. These data will open a novel approach to understanding genomic regulatory syntax through phylogenetic analysis, as well as a bridge to direct functional assessment of human disease-relevant loci.
Grant Summary
Cross-species analysis of regulatory function using synthetic regulatory genomics is a NHGRI - National Human Genome Research Institute grant providing up to $466K for university, nonprofit, healthcare org. Applications are due 2028-04-30 (open). Check eligibility and apply with FindGrants.
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How to Apply
Up to $466K
2028-04-30
- 1Confirm your organization is eligible for Cross-species analysis of regulatory function using synthetic regulatory genomics from NHGRI - National Human Genome Research Institute, checking organization type, location, and any population or project requirements.
- 2Gather the required documents and information, including your organization details, project plan, and budget figures.
- 3Draft your application narrative and budget addressing the funder's priorities and review criteria. FindGrants can draft each section for you to review and edit.
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Cross-species analysis of regulatory function using synthetic regulatory genomics: Frequently Asked Questions
Who is eligible for the Cross-species analysis of regulatory function using synthetic regulatory genomics?
Cross-species analysis of regulatory function using synthetic regulatory genomics is offered by NHGRI - National Human Genome Research Institute and is generally open to university, nonprofit, healthcare org. It is open to organizations nationwide unless the funder specifies otherwise. Review the specific eligibility terms before applying, since funders set their own requirements around organization type, location, and the population or project being served.
How much funding does the Cross-species analysis of regulatory function using synthetic regulatory genomics provide?
Cross-species analysis of regulatory function using synthetic regulatory genomics provides up to $466K per award from NHGRI - National Human Genome Research Institute. Actual award sizes depend on the scope of your project, available program funds, and the number of applicants, so build a budget that reflects realistic, allowable costs rather than the maximum figure.
When is the Cross-species analysis of regulatory function using synthetic regulatory genomics deadline?
Applications for Cross-species analysis of regulatory function using synthetic regulatory genomics are due 2028-04-30 (open). Because deadlines can change, verify the date with the funder, NHGRI - National Human Genome Research Institute, and give yourself enough time to prepare a complete, competitive application before the close date.
How do you apply for the Cross-species analysis of regulatory function using synthetic regulatory genomics?
To apply for Cross-species analysis of regulatory function using synthetic regulatory genomics, confirm your eligibility, gather the required documents, and prepare a narrative and budget that address the funder's priorities. FindGrants guides you step by step and can draft each section, then exports a submission-ready application pack for this grant from NHGRI - National Human Genome Research Institute.