Lysine Methylation as a Novel Regulatory Mechanism of HPV E2 Function
NIAID - National Institute of Allergy and Infectious Diseases
About This Grant
PROJECT ABSTRACT Human papillomaviruses (HPV) are double-stranded DNA viruses that infect cutaneous and mucosal epithelia. Infections with high-risk HPVs may lead to cervical, anogenital, and oropharyngeal cancers accounting for 5% of all cancers worldwide. Currently there are no effective non-invasive treatments against high-risk HPV infections. Because of that, it is critical to study the fundamental biology of high-risk HPV and specifically its utilization of host cell factors to permit completion of its viral life cycle. The viral replicative program consists of three different stages: (1) initial replication after infection, (2) stable maintenance of viral genomes and (3) amplification of viral episomes. The PV activator E2 and E1 DNA helicase proteins are the first proteins to be expressed after infection and are essential for HPV replication. The viral E2 protein is necessary for replication of the viral genome, activation of the viral early promoter for transcription, and tethering of viral genomes to host mitotic chromosomes in basal cells during cellular replication and division. Our long-term goal is to elucidate host factors necessary for E1-E2 dependent HPV replication and E2-mediated transcription throughout the viral lifecycle. Protein lysine methylation is emerging as an important post-translational modification that regulates the activity of transcription factors and histones. We recently identified four lysine residues methylated within the HPV-31 E2 by mass spectrometry confirming that E2 is a methylated protein. We hypothesize that that lysine methylation is an important regulatory process during the viral lifecycle. The overall objectives in this application are to (i) identify the lysine methyl transferases responsible for E2 methylation and (ii) determine their biological function. The proposed research is significant because it will define the mechanisms tightly controlled by E2 methylation throughout the HPV lifecycle. Ultimately, such knowledge has the potential to target mechanisms of persistent HPV infections.
Grant Summary
Lysine Methylation as a Novel Regulatory Mechanism of HPV E2 Function is a NIAID - National Institute of Allergy and Infectious Diseases grant providing up to $209K for university, nonprofit, healthcare org. Applications are due 2028-04-30 (open). Check eligibility and apply with FindGrants.
Focus Areas
Eligibility
How to Apply
Up to $209K
2028-04-30
- 1Confirm your organization is eligible for Lysine Methylation as a Novel Regulatory Mechanism of HPV E2 Function from NIAID - National Institute of Allergy and Infectious Diseases, checking organization type, location, and any population or project requirements.
- 2Gather the required documents and information, including your organization details, project plan, and budget figures.
- 3Draft your application narrative and budget addressing the funder's priorities and review criteria. FindGrants can draft each section for you to review and edit.
- 4Review every section against the requirements checklist, then export a submission-ready application pack and submit it to NIAID - National Institute of Allergy and Infectious Diseases before the deadline.
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Lysine Methylation as a Novel Regulatory Mechanism of HPV E2 Function: Frequently Asked Questions
Who is eligible for the Lysine Methylation as a Novel Regulatory Mechanism of HPV E2 Function?
Lysine Methylation as a Novel Regulatory Mechanism of HPV E2 Function is offered by NIAID - National Institute of Allergy and Infectious Diseases and is generally open to university, nonprofit, healthcare org. It is open to organizations nationwide unless the funder specifies otherwise. Review the specific eligibility terms before applying, since funders set their own requirements around organization type, location, and the population or project being served.
How much funding does the Lysine Methylation as a Novel Regulatory Mechanism of HPV E2 Function provide?
Lysine Methylation as a Novel Regulatory Mechanism of HPV E2 Function provides up to $209K per award from NIAID - National Institute of Allergy and Infectious Diseases. Actual award sizes depend on the scope of your project, available program funds, and the number of applicants, so build a budget that reflects realistic, allowable costs rather than the maximum figure.
When is the Lysine Methylation as a Novel Regulatory Mechanism of HPV E2 Function deadline?
Applications for Lysine Methylation as a Novel Regulatory Mechanism of HPV E2 Function are due 2028-04-30 (open). Because deadlines can change, verify the date with the funder, NIAID - National Institute of Allergy and Infectious Diseases, and give yourself enough time to prepare a complete, competitive application before the close date.
How do you apply for the Lysine Methylation as a Novel Regulatory Mechanism of HPV E2 Function?
To apply for Lysine Methylation as a Novel Regulatory Mechanism of HPV E2 Function, confirm your eligibility, gather the required documents, and prepare a narrative and budget that address the funder's priorities. FindGrants guides you step by step and can draft each section, then exports a submission-ready application pack for this grant from NIAID - National Institute of Allergy and Infectious Diseases.