Discovery and preliminary characterization of proteins required for Mycobacterium tuberculosis heme degradation
NIAID - National Institute of Allergy and Infectious Diseases
About This Grant
Tuberculosis (TB) continues to kill millions of people around the world, and the pathogenic bacteria Mycobacterium tuberculosis (Mtb) is the causative agent of TB. New tools to prevent and treat this deadly disease are urgently needed. Iron is an essential metal for all forms of life and most bacterial pathogens including Mtb must import iron from its host to survive. Therefore, Mtb iron uptake pathways and their regulation may provide good targets for therapeutic development. We will describe the further characterization of a protein in the Mtb heme uptake pathway that catalyzes the release of iron from heme. This proposal aims to discover the additional required proteins in Mtb heme catabolism. The final step in the Mtb heme acquisition pathway is cytosolic heme degradation by the IsdG-type protein, MhuD. Canonical human heme oxygenases (HOs) are distinct both in sequence and structure from IsdG-type proteins. Unlike HOs, there is still a knowledge gap in the fate of the IsdG-type protein tetrapyrrole products. The high affinity of IsdG-type proteins for their tetrapyrrole products suggests that there is an additional protein in the heme uptake network that is responsible for product extraction from IsdG-type proteins. We propose that like HOs, the protein that extracts and reduces the MhuD product is one of the putative Mtb biliverdin reductases (BVRs), which are also sequence and structure distinct from human BVRs. Another unknown protein involved in MhuD heme degradation is the protein that provides the electron source to facilitate the reaction. We aim to identify these proteins of interest by discovery-based techniques. Utilizing biochemical and microbiology techniques, this proposal will investigate the fate of MhuD tetrapyrrole products and discover the protein that provides electrons for MhuD heme degradation. The results of this study will (1) lay the groundwork to further explore the downstream steps for MhuD heme degradation, and (2) identify unique Mtb targets for therapeutic development.
Grant Summary
Discovery and preliminary characterization of proteins required for Mycobacterium tuberculosis heme degradation is a NIAID - National Institute of Allergy and Infectious Diseases grant providing up to $205K for university, nonprofit, healthcare org. Applications are due 2028-06-30 (open). Check eligibility and apply with FindGrants.
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How to Apply
Up to $205K
2028-06-30
- 1Confirm your organization is eligible for Discovery and preliminary characterization of proteins required for Mycobacterium tuberculosis heme degradation from NIAID - National Institute of Allergy and Infectious Diseases, checking organization type, location, and any population or project requirements.
- 2Gather the required documents and information, including your organization details, project plan, and budget figures.
- 3Draft your application narrative and budget addressing the funder's priorities and review criteria. FindGrants can draft each section for you to review and edit.
- 4Review every section against the requirements checklist, then export a submission-ready application pack and submit it to NIAID - National Institute of Allergy and Infectious Diseases before the deadline.
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Discovery and preliminary characterization of proteins required for Mycobacterium tuberculosis heme degradation: Frequently Asked Questions
Who is eligible for the Discovery and preliminary characterization of proteins required for Mycobacterium tuberculosis heme degradation?
Discovery and preliminary characterization of proteins required for Mycobacterium tuberculosis heme degradation is offered by NIAID - National Institute of Allergy and Infectious Diseases and is generally open to university, nonprofit, healthcare org. It is open to organizations nationwide unless the funder specifies otherwise. Review the specific eligibility terms before applying, since funders set their own requirements around organization type, location, and the population or project being served.
How much funding does the Discovery and preliminary characterization of proteins required for Mycobacterium tuberculosis heme degradation provide?
Discovery and preliminary characterization of proteins required for Mycobacterium tuberculosis heme degradation provides up to $205K per award from NIAID - National Institute of Allergy and Infectious Diseases. Actual award sizes depend on the scope of your project, available program funds, and the number of applicants, so build a budget that reflects realistic, allowable costs rather than the maximum figure.
When is the Discovery and preliminary characterization of proteins required for Mycobacterium tuberculosis heme degradation deadline?
Applications for Discovery and preliminary characterization of proteins required for Mycobacterium tuberculosis heme degradation are due 2028-06-30 (open). Because deadlines can change, verify the date with the funder, NIAID - National Institute of Allergy and Infectious Diseases, and give yourself enough time to prepare a complete, competitive application before the close date.
How do you apply for the Discovery and preliminary characterization of proteins required for Mycobacterium tuberculosis heme degradation?
To apply for Discovery and preliminary characterization of proteins required for Mycobacterium tuberculosis heme degradation, confirm your eligibility, gather the required documents, and prepare a narrative and budget that address the funder's priorities. FindGrants guides you step by step and can draft each section, then exports a submission-ready application pack for this grant from NIAID - National Institute of Allergy and Infectious Diseases.