Deciphering Phagosomal Membrane Repair Pathways during Mycobacterium tuberculosis Infection Using High-Throughput Single Cell and Single Phagosome Analysis
NIAID - National Institute of Allergy and Infectious Diseases
About This Grant
Phagosomal membrane repair is a cellular process that prevents pathogen escape and host cell death, which are key to the virulence of intracellular pathogens such as Mycobacterium tuberculosis (Mtb). Different effectors and pathways contribute to the repair of the phagosome, such as the ESCRT machinery and an endoplasmic reticulum-dependent membrane repair mechanism. Concomitantly, the induction of damages on the phagosome triggers autophagy, which is also suspected to contribute to the repair of the phagosome. The knowledge of the host cell mechanisms of phagosome damage and repair in Mtb-infected human macrophages is incomplete. Our previous results have shown dynamic recruitment of LC3 to damaged phagosomal membranes, and the loss of Lysoview staining as a readout for membrane damage, indicating active repair processes. Time-lapse microscopy has proven to be an efficient method to directly visualize and decipher membrane damage and repair of the Mycobacterium-Containing Vacuole (MCV). In the first specific aim (SA1) we will expand upon our initial analysis of the spatio-temporal analysis of MCV membrane repair by including novel host cell marker proteins, investigate the process in primary human monocyte-derived macrophages (hMDMs) and develop novel bioinformatics tools to improve image analysis. In SA2 we will perform a targeted genetic screen for host proteins involved in MCV membrane repair via siRNA knock-down in THP-1 cells and the results will be confirmed in hMDMs. SA3 will investigate the phagosomal membrane damage and repair dynamics in response to different mycobacterial species which differ in their virulence factors involved in inducing phagosomal damage.
Grant Summary
Deciphering Phagosomal Membrane Repair Pathways during Mycobacterium tuberculosis Infection Using High-Throughput Single Cell and Single Phagosome Analysis is a NIAID - National Institute of Allergy and Infectious Diseases grant providing up to $234K for university, nonprofit, healthcare org. Applications are due 2028-04-30 (open). Check eligibility and apply with FindGrants.
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Up to $234K
2028-04-30
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- 2Gather the required documents and information, including your organization details, project plan, and budget figures.
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Deciphering Phagosomal Membrane Repair Pathways during Mycobacterium tuberculosis Infection Using High-Throughput Single Cell and Single Phagosome Analysis: Frequently Asked Questions
Who is eligible for the Deciphering Phagosomal Membrane Repair Pathways during Mycobacterium tuberculosis Infection Using High-Throughput Single Cell and Single Phagosome Analysis?
Deciphering Phagosomal Membrane Repair Pathways during Mycobacterium tuberculosis Infection Using High-Throughput Single Cell and Single Phagosome Analysis is offered by NIAID - National Institute of Allergy and Infectious Diseases and is generally open to university, nonprofit, healthcare org. It is open to organizations nationwide unless the funder specifies otherwise. Review the specific eligibility terms before applying, since funders set their own requirements around organization type, location, and the population or project being served.
How much funding does the Deciphering Phagosomal Membrane Repair Pathways during Mycobacterium tuberculosis Infection Using High-Throughput Single Cell and Single Phagosome Analysis provide?
Deciphering Phagosomal Membrane Repair Pathways during Mycobacterium tuberculosis Infection Using High-Throughput Single Cell and Single Phagosome Analysis provides up to $234K per award from NIAID - National Institute of Allergy and Infectious Diseases. Actual award sizes depend on the scope of your project, available program funds, and the number of applicants, so build a budget that reflects realistic, allowable costs rather than the maximum figure.
When is the Deciphering Phagosomal Membrane Repair Pathways during Mycobacterium tuberculosis Infection Using High-Throughput Single Cell and Single Phagosome Analysis deadline?
Applications for Deciphering Phagosomal Membrane Repair Pathways during Mycobacterium tuberculosis Infection Using High-Throughput Single Cell and Single Phagosome Analysis are due 2028-04-30 (open). Because deadlines can change, verify the date with the funder, NIAID - National Institute of Allergy and Infectious Diseases, and give yourself enough time to prepare a complete, competitive application before the close date.
How do you apply for the Deciphering Phagosomal Membrane Repair Pathways during Mycobacterium tuberculosis Infection Using High-Throughput Single Cell and Single Phagosome Analysis?
To apply for Deciphering Phagosomal Membrane Repair Pathways during Mycobacterium tuberculosis Infection Using High-Throughput Single Cell and Single Phagosome Analysis, confirm your eligibility, gather the required documents, and prepare a narrative and budget that address the funder's priorities. FindGrants guides you step by step and can draft each section, then exports a submission-ready application pack for this grant from NIAID - National Institute of Allergy and Infectious Diseases.