Identification of Immune Activation DNA inside Cells
NIAID - National Institute of Allergy and Infectious Diseases
About This Grant
Identification of immune activation DNA inside cells Cyclic GMP-AMP synthase (cGAS) is a key sensor of cytosolic double-stranded DNA (dsDNA) that activates the STimulator of INterferon Genes (STING) pathway, triggering immune responses against infections and cellular damage. While cGAS plays a protective role in pathogen defense and tumor suppression, its aberrant activation by self-DNA is implicated in autoimmune diseases (e.g., Aicardi- Goutières Syndrome, systemic lupus erythematosus) and chronic inflammation associated with aging. Despite extensive studies on cGAS-STING signaling, the physiological dsDNA ligands that activate cGAS under various cellular conditions remain poorly characterized due to the transient and weak nature of cGAS-DNA interactions, which conventional crosslinking methods fail to capture. To address this challenge, we propose to develop and apply a novel photochemical crosslinking approach to covalently capture cGAS-DNA complexes in living cells. Aim 1 focuses on adapting and optimizing this crosslinking technology for cGAS-DNA interactions, ensuring high efficiency and specificity. Aim 2 applies this method to establish a first-in-class cGAS BFPX ChIP-seq protocol, enabling the genome-wide identification of endogenous DNA ligands that activate cGAS in Trex1 KO MEF cells—a cellular model of Aicardi- Goutières Syndrome. This innovative approach will provide a powerful tool for studying cGAS-STING pathway activation across diverse physiological and pathological contexts. By uncovering the sources and mechanisms of immune-activating DNA, our work will pave the way for novel diagnostic and therapeutic strategies targeting cGAS in autoimmune, inflammatory, and age-related diseases.
Grant Summary
Identification of Immune Activation DNA inside Cells is a NIAID - National Institute of Allergy and Infectious Diseases grant providing up to $457K for university, nonprofit, healthcare org. Applications are due 2028-01-31 (open). Check eligibility and apply with FindGrants.
Focus Areas
Eligibility
How to Apply
Up to $457K
2028-01-31
- 1Confirm your organization is eligible for Identification of Immune Activation DNA inside Cells from NIAID - National Institute of Allergy and Infectious Diseases, checking organization type, location, and any population or project requirements.
- 2Gather the required documents and information, including your organization details, project plan, and budget figures.
- 3Draft your application narrative and budget addressing the funder's priorities and review criteria. FindGrants can draft each section for you to review and edit.
- 4Review every section against the requirements checklist, then export a submission-ready application pack and submit it to NIAID - National Institute of Allergy and Infectious Diseases before the deadline.
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Identification of Immune Activation DNA inside Cells: Frequently Asked Questions
Who is eligible for the Identification of Immune Activation DNA inside Cells?
Identification of Immune Activation DNA inside Cells is offered by NIAID - National Institute of Allergy and Infectious Diseases and is generally open to university, nonprofit, healthcare org. It is open to organizations nationwide unless the funder specifies otherwise. Review the specific eligibility terms before applying, since funders set their own requirements around organization type, location, and the population or project being served.
How much funding does the Identification of Immune Activation DNA inside Cells provide?
Identification of Immune Activation DNA inside Cells provides up to $457K per award from NIAID - National Institute of Allergy and Infectious Diseases. Actual award sizes depend on the scope of your project, available program funds, and the number of applicants, so build a budget that reflects realistic, allowable costs rather than the maximum figure.
When is the Identification of Immune Activation DNA inside Cells deadline?
Applications for Identification of Immune Activation DNA inside Cells are due 2028-01-31 (open). Because deadlines can change, verify the date with the funder, NIAID - National Institute of Allergy and Infectious Diseases, and give yourself enough time to prepare a complete, competitive application before the close date.
How do you apply for the Identification of Immune Activation DNA inside Cells?
To apply for Identification of Immune Activation DNA inside Cells, confirm your eligibility, gather the required documents, and prepare a narrative and budget that address the funder's priorities. FindGrants guides you step by step and can draft each section, then exports a submission-ready application pack for this grant from NIAID - National Institute of Allergy and Infectious Diseases.