Identification of placental iron-regulatory hormones
NIDDK - National Institute of Diabetes and Digestive and Kidney Diseases
About This Grant
PROJECT SUMMARY Iron is essential for a healthy pregnancy. During pregnancy, iron requirements increase substantially to support the development of the placenta and fetus as well as maternal blood cell volume expansion. Insufficient iron and ensuing iron deficiency anemia is linked to adverse pregnancy outcomes, including increased maternal mortality, perinatal death, and preterm birth. Hepcidin regulates systemic iron homeostasis by controlling dietary iron absorption, the release of iron from iron recycling macrophages, and the release of iron from hepatic stores. During pregnancy in humans and rodents, maternal hepcidin is profoundly suppressed, which is thought to maximize dietary iron absorption and mobilization of iron from stores for transfer to the developing fetus. Augmenting maternal hepcidin in mouse pregnancy by administration of hepcidin analogs led to severe embryo anemia or even death. Thus, maternal hepcidin suppression is essential for maternal and embryo iron homeostasis and health. Despite its importance, the mechanism(s) responsible for hepcidin suppression remain elusive. The goal of this project is to identify the pregnancy-related hepcidin suppressor. Specific Aim 1. Identify novel hepcidin regulators produced by the trophoblast – We identified over 600 proteins in our LC-MS/MS analysis of hepcidin suppressive fractions purified from human trophoblast supernatant and placental interstitial fluid. We will perform a high throughput cDNA overexpression screen of individual candidates to identify all proteins that modulate hepcidin expression in hepatocytes. Specific Aim 2. Investigate the role of HGF in hepcidin suppression during pregnancy – Our lab previously discovered that HGF suppresses hepcidin in vitro, but the physiological context for the role of HGF in iron metabolism was lacking. We now detected HGF in the placental LC-MS/MS screen and will characterize its role in hepcidin suppression in vitro and in vivo. In vitro, we will i) deplete HGF from suppressive trophoblast-derived conditioned media and placental interstitial fluid and ii) inhibit HGF signaling in hepatocytes treated with trophoblast media and placental interstitial fluid, and in each case assess their ability to suppress hepcidin. In vivo, we will i) measure HGF concentrations and the proportion of pro- HGF and mature-HGF in maternal circulation across gestation and ii) inhibit HGF receptor MET at the gestational age when maternal hepcidin suppression is initiated and assess hepcidin suppression and serum iron changes. When completed, these studies will provide fundamental insight into the regulation of iron homeostasis during and even outside of pregnancy, with broad translational potential for treatment of iron disorders.
Grant Summary
Identification of placental iron-regulatory hormones is a NIDDK - National Institute of Diabetes and Digestive and Kidney Diseases grant providing up to $118K for university, nonprofit, healthcare org. Applications are due 2028-01-31 (open). Check eligibility and apply with FindGrants.
Focus Areas
Eligibility
How to Apply
Up to $118K
2028-01-31
- 1Confirm your organization is eligible for Identification of placental iron-regulatory hormones from NIDDK - National Institute of Diabetes and Digestive and Kidney Diseases, checking organization type, location, and any population or project requirements.
- 2Gather the required documents and information, including your organization details, project plan, and budget figures.
- 3Draft your application narrative and budget addressing the funder's priorities and review criteria. FindGrants can draft each section for you to review and edit.
- 4Review every section against the requirements checklist, then export a submission-ready application pack and submit it to NIDDK - National Institute of Diabetes and Digestive and Kidney Diseases before the deadline.
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Identification of placental iron-regulatory hormones: Frequently Asked Questions
Who is eligible for the Identification of placental iron-regulatory hormones?
Identification of placental iron-regulatory hormones is offered by NIDDK - National Institute of Diabetes and Digestive and Kidney Diseases and is generally open to university, nonprofit, healthcare org. It is open to organizations nationwide unless the funder specifies otherwise. Review the specific eligibility terms before applying, since funders set their own requirements around organization type, location, and the population or project being served.
How much funding does the Identification of placental iron-regulatory hormones provide?
Identification of placental iron-regulatory hormones provides up to $118K per award from NIDDK - National Institute of Diabetes and Digestive and Kidney Diseases. Actual award sizes depend on the scope of your project, available program funds, and the number of applicants, so build a budget that reflects realistic, allowable costs rather than the maximum figure.
When is the Identification of placental iron-regulatory hormones deadline?
Applications for Identification of placental iron-regulatory hormones are due 2028-01-31 (open). Because deadlines can change, verify the date with the funder, NIDDK - National Institute of Diabetes and Digestive and Kidney Diseases, and give yourself enough time to prepare a complete, competitive application before the close date.
How do you apply for the Identification of placental iron-regulatory hormones?
To apply for Identification of placental iron-regulatory hormones, confirm your eligibility, gather the required documents, and prepare a narrative and budget that address the funder's priorities. FindGrants guides you step by step and can draft each section, then exports a submission-ready application pack for this grant from NIDDK - National Institute of Diabetes and Digestive and Kidney Diseases.