Decoding the glycan forms of glycoRNA-L and glycoRNA-S from leukocytes
NIAID - National Institute of Allergy and Infectious Diseases
About This Grant
Project Summary In mammalian cells, RNAs are predominantly located within cytoplasm and nucleus. A recent study, however, has reported that RNAs can be glycosylated, and such glycosylated RNAs (called glycoRNAs) are localized on out cell surface. The glycoRNAs are glycan-modified small non-coding RNAs. These RNAs are transcribed and maturated in nucleus and transported to cytoplasm for processing and then conjugated with glycan chains in endoplasmic reticulum. The conjugated molecules are then transported to cell membrane and anchored on cell membrane by binding to transmembrane RNA binding proteins. The cell surface glycoRNAs can serve as ligands or receptors to mediate cell-cell interaction, communication and infiltration and may also trigger cell signal transductions. Our recent works suggest that there were two forms of glycoRNAs, named glycoRNA-L and glycoRNA-S, robustly expressed in leukocytes, but not non-immune cells. GlycoRNA-L was migrated with ~11 kb RNA, whereas glycoRNA-S was migrated with ~0.6 kb RNAs. Using RNase removed the RNA frag- ments, the migration of both glycoRNA-L and glycoRNA-S was slightly faster, suggesting that the migration of glycoRNA-L and glycoRNA-S was determined by the glycan chains, but not RNA fragments. Further studies suggest that the glycan structures and functions of glycoRNA-L and glycoRNA-S may be distinguished. How- ever, the compositions and structures of glycan forms of glycoRNA-L and glycoRNA-S remain unknown. We hypothesize that the glycoforms of glycoRNA-L and glycoRNA-S from leukocytes are determinants of their functional significance. The goal of this proposal is to decode the compositions and structures of glycan forms of glycoRNA-L and glycoRNA-S from leukocytes. We will reach this goal by two approaches: 1) metabolic labeling combined with enzymatic digestion; 2) glycoRNA-L and glycoRNA-S purification and LC/MS analysis. Completion of the project will significantly advance our understanding of the role and mechanisms of glycoRNA- L and glycoRNA-S in the regulation of immune response and immune-related human diseases. The study will also provide a method for mapping the glycan structures of glycoRNAs from other cells such as cancer cells, which may provide insights for generating innovative diagnostic and therapeutic strategies.
Grant Summary
Decoding the glycan forms of glycoRNA-L and glycoRNA-S from leukocytes is a NIAID - National Institute of Allergy and Infectious Diseases grant providing up to $158K for university, nonprofit, healthcare org. Applications are due 2028-03-31 (open). Check eligibility and apply with FindGrants.
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Up to $158K
2028-03-31
- 1Confirm your organization is eligible for Decoding the glycan forms of glycoRNA-L and glycoRNA-S from leukocytes from NIAID - National Institute of Allergy and Infectious Diseases, checking organization type, location, and any population or project requirements.
- 2Gather the required documents and information, including your organization details, project plan, and budget figures.
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Decoding the glycan forms of glycoRNA-L and glycoRNA-S from leukocytes: Frequently Asked Questions
Who is eligible for the Decoding the glycan forms of glycoRNA-L and glycoRNA-S from leukocytes?
Decoding the glycan forms of glycoRNA-L and glycoRNA-S from leukocytes is offered by NIAID - National Institute of Allergy and Infectious Diseases and is generally open to university, nonprofit, healthcare org. It is open to organizations nationwide unless the funder specifies otherwise. Review the specific eligibility terms before applying, since funders set their own requirements around organization type, location, and the population or project being served.
How much funding does the Decoding the glycan forms of glycoRNA-L and glycoRNA-S from leukocytes provide?
Decoding the glycan forms of glycoRNA-L and glycoRNA-S from leukocytes provides up to $158K per award from NIAID - National Institute of Allergy and Infectious Diseases. Actual award sizes depend on the scope of your project, available program funds, and the number of applicants, so build a budget that reflects realistic, allowable costs rather than the maximum figure.
When is the Decoding the glycan forms of glycoRNA-L and glycoRNA-S from leukocytes deadline?
Applications for Decoding the glycan forms of glycoRNA-L and glycoRNA-S from leukocytes are due 2028-03-31 (open). Because deadlines can change, verify the date with the funder, NIAID - National Institute of Allergy and Infectious Diseases, and give yourself enough time to prepare a complete, competitive application before the close date.
How do you apply for the Decoding the glycan forms of glycoRNA-L and glycoRNA-S from leukocytes?
To apply for Decoding the glycan forms of glycoRNA-L and glycoRNA-S from leukocytes, confirm your eligibility, gather the required documents, and prepare a narrative and budget that address the funder's priorities. FindGrants guides you step by step and can draft each section, then exports a submission-ready application pack for this grant from NIAID - National Institute of Allergy and Infectious Diseases.