Deconvolving Vertebrate Hox Binding Preferences and Activation Properties
NINDS - National Institute of Neurological Disorders and Stroke
About This Grant
PROJECT SUMMARY Embryonic development culminates with an astonishing number of terminal differentiation cellular states. Transcription factors (TFs) bind to specific DNA sequences in the genome and are key regulators of gene transcription responsible for generating cell diversity. TFs are grouped in families with similar DNA binding preferences and sometimes similar activation or repression domains, yet they perform different functions during cell differentiation. Consequently, how different TFs of the same family bind and regulate gene expression is poorly understood. Hox genes code for homeodomain (HD) TFs, the second largest TF family in the mammalian genome. Vertebrate HOX TFs are divided into anterior (HOX1-5), central (HOX6-8), and posterior (HOX9-13) paralog groups. In vertebrates, Hox genes pattern various developing tissues. Notably, spinal cord neuronal diversity requires Hox gene activity along its rostro-caudal axis. The role of Hox genes in organizing the spinal cord presents an interesting conundrum. The limb-innervating expression program is controlled by central (HOX6 & HOX8) HOX TFs at the brachial level and posterior (HOX10) HOX TFs at the lumbar spinal cord. Thus, HOX TFs with different DNA sequence preferences induce a similar motor neuron fate. Meanwhile, the posterior HOXC9 induces thoracic fate. Thus, two posterior group genes, Hoxc9 and Hoxc10, induce different spinal cord fates. In agreement with their genomic cluster position, Hox13 paralogs are expressed late during development, distally, and in posterior regions. This application aims to understand how the mammalian posterior group Hox TFs diversify their function to pattern the spinal cord. Aim 1 will dwell more on the protein domain and specific HOX amino acids in Hoxc9 and Hoxc13 that mediate their ability to bind to inaccessible chromatin and DNA residence time. Aim 2 will take a complementary machine learning approach using ChIP-exo data coupled with chromatin features to distinguish individual pioneer Hox binding sites from non-pioneer sites. Aim 3 will investigate how the very variable N-terminus controls the transcriptional output.
Grant Summary
Deconvolving Vertebrate Hox Binding Preferences and Activation Properties is a NINDS - National Institute of Neurological Disorders and Stroke grant providing up to $639K for university, nonprofit, healthcare org. Applications are due 2031-04-30 (open). Check eligibility and apply with FindGrants.
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How to Apply
Up to $639K
2031-04-30
- 1Confirm your organization is eligible for Deconvolving Vertebrate Hox Binding Preferences and Activation Properties from NINDS - National Institute of Neurological Disorders and Stroke, checking organization type, location, and any population or project requirements.
- 2Gather the required documents and information, including your organization details, project plan, and budget figures.
- 3Draft your application narrative and budget addressing the funder's priorities and review criteria. FindGrants can draft each section for you to review and edit.
- 4Review every section against the requirements checklist, then export a submission-ready application pack and submit it to NINDS - National Institute of Neurological Disorders and Stroke before the deadline.
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Deconvolving Vertebrate Hox Binding Preferences and Activation Properties: Frequently Asked Questions
Who is eligible for the Deconvolving Vertebrate Hox Binding Preferences and Activation Properties?
Deconvolving Vertebrate Hox Binding Preferences and Activation Properties is offered by NINDS - National Institute of Neurological Disorders and Stroke and is generally open to university, nonprofit, healthcare org. It is open to organizations nationwide unless the funder specifies otherwise. Review the specific eligibility terms before applying, since funders set their own requirements around organization type, location, and the population or project being served.
How much funding does the Deconvolving Vertebrate Hox Binding Preferences and Activation Properties provide?
Deconvolving Vertebrate Hox Binding Preferences and Activation Properties provides up to $639K per award from NINDS - National Institute of Neurological Disorders and Stroke. Actual award sizes depend on the scope of your project, available program funds, and the number of applicants, so build a budget that reflects realistic, allowable costs rather than the maximum figure.
When is the Deconvolving Vertebrate Hox Binding Preferences and Activation Properties deadline?
Applications for Deconvolving Vertebrate Hox Binding Preferences and Activation Properties are due 2031-04-30 (open). Because deadlines can change, verify the date with the funder, NINDS - National Institute of Neurological Disorders and Stroke, and give yourself enough time to prepare a complete, competitive application before the close date.
How do you apply for the Deconvolving Vertebrate Hox Binding Preferences and Activation Properties?
To apply for Deconvolving Vertebrate Hox Binding Preferences and Activation Properties, confirm your eligibility, gather the required documents, and prepare a narrative and budget that address the funder's priorities. FindGrants guides you step by step and can draft each section, then exports a submission-ready application pack for this grant from NINDS - National Institute of Neurological Disorders and Stroke.