Strategies to promote the maturation of pre-hematopoietic stem cells (pre-HSCs) into HSCs
About This Grant
Summary The overarching goals of this proposal are to identify pathways necessary for the maturation of pre-hematopoietic stem cells (pre-HSCs) into long-term, multi-lineage adult-repopulating HSCs. Our primary focus is on the transforming growth factor beta (TGFβ) and/or bone morphogenetic protein (BMP) signaling pathways, based on our discovery that a negative regulator of these pathways, mothers against decapentaplegic homologue 7 (SMAD7), is important for efficient pre- HSC to HSC maturation in mouse embryos. Current stepwise protocols for producing long-term multi-lineage adult-repopulating HSCs from induced pluripotent stem cells (iPSCs) modulate TGFβ and BMP signaling at early stages of the culture, but not during the later pre-HSC to HSC maturation stage. We will use a quantitative ex vivo culture system to systematically interrogate which components of the TGFβ/BMP signaling pathway must be inhibited, and when, to improve HSC maturation from SMAD7-deficient pre-HSCs. We will also determine the molecular changes that occur over the course of pre-HSC to HSC maturation using clonal barcoding single-cell RNA sequencing to identify pre-HSCs that have undergone successful HSC maturation and develop transcriptional profiles of HSC-forming pre-HSCs throughout culture. Finally, we will apply the knowledge we gain by studying this process in mouse embryos to optimize the generation of HSCs iPSCs.
Grant Summary
Strategies to promote the maturation of pre-hematopoietic stem cells (pre-HSCs) into HSCs is a NHLBI - National Heart Lung and Blood Institute grant providing up to $633K for university, nonprofit, healthcare org. Applications are due 2030-05-31 (open). Check eligibility and apply with FindGrants.
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Focus Areas
Eligibility
How to Apply
Up to $633K
2030-05-31
- 1Confirm your organization is eligible for Strategies to promote the maturation of pre-hematopoietic stem cells (pre-HSCs) into HSCs from NHLBI - National Heart Lung and Blood Institute, checking organization type, location, and any population or project requirements.
- 2Gather the required documents and information, including your organization details, project plan, and budget figures.
- 3Draft your application narrative and budget addressing the funder's priorities and review criteria. FindGrants can draft each section for you to review and edit.
- 4Review every section against the requirements checklist, then export a submission-ready application pack and submit it to NHLBI - National Heart Lung and Blood Institute before the deadline.
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Strategies to promote the maturation of pre-hematopoietic stem cells (pre-HSCs) into HSCs: Frequently Asked Questions
Who is eligible for the Strategies to promote the maturation of pre-hematopoietic stem cells (pre-HSCs) into HSCs?
Strategies to promote the maturation of pre-hematopoietic stem cells (pre-HSCs) into HSCs is offered by NHLBI - National Heart Lung and Blood Institute and is generally open to university, nonprofit, healthcare org. It is open to organizations nationwide unless the funder specifies otherwise. Review the specific eligibility terms before applying, since funders set their own requirements around organization type, location, and the population or project being served.
How much funding does the Strategies to promote the maturation of pre-hematopoietic stem cells (pre-HSCs) into HSCs provide?
Strategies to promote the maturation of pre-hematopoietic stem cells (pre-HSCs) into HSCs provides up to $633K per award from NHLBI - National Heart Lung and Blood Institute. Actual award sizes depend on the scope of your project, available program funds, and the number of applicants, so build a budget that reflects realistic, allowable costs rather than the maximum figure.
When is the Strategies to promote the maturation of pre-hematopoietic stem cells (pre-HSCs) into HSCs deadline?
Applications for Strategies to promote the maturation of pre-hematopoietic stem cells (pre-HSCs) into HSCs are due 2030-05-31 (open). Because deadlines can change, verify the date with the funder, NHLBI - National Heart Lung and Blood Institute, and give yourself enough time to prepare a complete, competitive application before the close date.
How do you apply for the Strategies to promote the maturation of pre-hematopoietic stem cells (pre-HSCs) into HSCs?
To apply for Strategies to promote the maturation of pre-hematopoietic stem cells (pre-HSCs) into HSCs, confirm your eligibility, gather the required documents, and prepare a narrative and budget that address the funder's priorities. FindGrants guides you step by step and can draft each section, then exports a submission-ready application pack for this grant from NHLBI - National Heart Lung and Blood Institute.