Investigating the role of the human milk metabolome and microRNAs on metabolic health in breastfeeding children with and without perinatal HIV exposure
NICHD - Eunice Kennedy Shriver National Institute of Child Health and Human Development
About This Grant
PROJECT SUMMARY More than 39.9 million people are living with HIV worldwide, including 1.2 million women who become pregnant annually. Suppressive antiretroviral therapy (ART) to pregnant women with HIV (WHIV) has been highly successful in reducing the number of new pediatric infections. However, HIV/ART exposure in utero and infancy can lead to reduced early life growth predisposing children for lifelong metabolic disease risk, so understanding adverse effects of HIV/ART exposure on infants is critical to protect millions of children worldwide. Human milk (HM) is essential for early infant metabolic health, and breastfeeding is recommended globally for WHIV on ART. However, HM is a complex mixture that is highly dependent on the maternal environment and HM components may influence child health. HM is rich in extracellular vesicles (EVs), nano-sized packages that survive digestion to deliver their bioactive cargoes. EV miRNAs (small noncoding RNAs) and metabolites (small polar metabolites and lipids) are important bioactive molecules that hold promise as drivers of these effects. Our goal is to investigate the role of maternal HIV/ART exposure on the HM EV-metabolome and EV-miRNAs and their subsequent impacts on child growth and body composition. To address this, we propose to leverage the Africa-based Obesogenic oRigins of maternal and Child metabolic Health Involving Dolutegravir (ORCHID) study of pregnant WHIV receiving tenofovir + lamivudine + dolutegravir (TLD) and HIV-seronegative pregnant women and their children (analyzing a subset, N=500; 250/group). Using anthropometry at 1, 2, 3, 6, 12 and 24 months of age and gold standard air displacement plethysmography to assess body composition, we will construct trajectories of overall infant growth and adiposity. In HM samples collected at 1 and 3 months postpartum, we will evaluate EV-miRNA expression with next-generation sequencing and the HM EV-metabolome with ultrasensitive metabolomics and lipidomics to address the following aims: Aim 1. Assess the association of maternal HIV/ART exposure with the human milk EV-metabolome and EV-miRNA across lactation; Aim 2. Investigate the associations of the human milk EV-metabolome and EV-miRNA with child growth; and Exploratory Aim 3. Develop a predictive multi-omic fingerprint of milk EV cargoes that can be used to identify HIV-exposed uninfected children (CHEU) with elevated risk of reduced growth and adiposity. Using machine learning approaches that account for joint effects and interactions, we will characterize miRNA-metabolome profiles in combination with traditional risk factors that identify children with elevated metabolic risk due to HIV/ART exposure during breastfeeding. With more than 1 million WHIV becoming pregnant each year and increasing numbers of children being exposed to HIV/ART through breastfeeding, our work will provide critical knowledge on how HIV/ART exposure impacts breastmilk composition and maternal-child communication to affect child health, providing key insights on nutrition and future targets for intervention and prevention.
Grant Summary
Investigating the role of the human milk metabolome and microRNAs on metabolic health in breastfeeding children with and without perinatal HIV exposure is a NICHD - Eunice Kennedy Shriver National Institute of Child Health and Human Development grant providing up to $2.5M for university, nonprofit, healthcare org. Applications are due 2029-06-30 (open). Check eligibility and apply with FindGrants.
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Up to $2.5M
2029-06-30
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- 2Gather the required documents and information, including your organization details, project plan, and budget figures.
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Investigating the role of the human milk metabolome and microRNAs on metabolic health in breastfeeding children with and without perinatal HIV exposure: Frequently Asked Questions
Who is eligible for the Investigating the role of the human milk metabolome and microRNAs on metabolic health in breastfeeding children with and without perinatal HIV exposure?
Investigating the role of the human milk metabolome and microRNAs on metabolic health in breastfeeding children with and without perinatal HIV exposure is offered by NICHD - Eunice Kennedy Shriver National Institute of Child Health and Human Development and is generally open to university, nonprofit, healthcare org. It is open to organizations nationwide unless the funder specifies otherwise. Review the specific eligibility terms before applying, since funders set their own requirements around organization type, location, and the population or project being served.
How much funding does the Investigating the role of the human milk metabolome and microRNAs on metabolic health in breastfeeding children with and without perinatal HIV exposure provide?
Investigating the role of the human milk metabolome and microRNAs on metabolic health in breastfeeding children with and without perinatal HIV exposure provides up to $2.5M per award from NICHD - Eunice Kennedy Shriver National Institute of Child Health and Human Development. Actual award sizes depend on the scope of your project, available program funds, and the number of applicants, so build a budget that reflects realistic, allowable costs rather than the maximum figure.
When is the Investigating the role of the human milk metabolome and microRNAs on metabolic health in breastfeeding children with and without perinatal HIV exposure deadline?
Applications for Investigating the role of the human milk metabolome and microRNAs on metabolic health in breastfeeding children with and without perinatal HIV exposure are due 2029-06-30 (open). Because deadlines can change, verify the date with the funder, NICHD - Eunice Kennedy Shriver National Institute of Child Health and Human Development, and give yourself enough time to prepare a complete, competitive application before the close date.
How do you apply for the Investigating the role of the human milk metabolome and microRNAs on metabolic health in breastfeeding children with and without perinatal HIV exposure?
To apply for Investigating the role of the human milk metabolome and microRNAs on metabolic health in breastfeeding children with and without perinatal HIV exposure, confirm your eligibility, gather the required documents, and prepare a narrative and budget that address the funder's priorities. FindGrants guides you step by step and can draft each section, then exports a submission-ready application pack for this grant from NICHD - Eunice Kennedy Shriver National Institute of Child Health and Human Development.