Cost-effective workflow for efficient unsaturated lipidomics enabled by a novel ion chemistry
About This Grant
Abstract Isomerism in carbon-carbon double bond (C=C) position is common in unsaturated lipids. Tracking shifting lipid C=C isomerism in lipids not only sheds light on the complexity of the biological lipidome but also enables the detection of minor, yet significant, perturbations in lipid isomer ratios caused by certain disease conditions. Despite the significant progress made to enable resolution of lipid C=C positions, the existing technologies have their limitations, making it still challenging to do C=C isomeric lipid analysis in a cost-effective and high throughput manner. We recently discovered a novel ion chemistry, OzNOxESI, in the ionization source of a mass spectrometer. The reaction is sufficiently fast for coupling with LC-MS based lipidomics workflows, and the OzNOx adducts of unsaturated lipids produce ions diagnostic of C=C position under collisional induced dissociation, which provides a simple, easily accessible, and cost-effective solution for determining lipid C=C position with minimal modification to a mass spectrometer. In this project, we plan to extend this novel ion chemistry beyond phospholipids to be applicable to all lipid classes, particularly the structurally more complex glycerolipids and sphingolipids, and investigate how the intensity of C=C diagnostic ions changes with respect to location on the fatty acyl chain, aiming for an algorithm that can correct C=C position induced-ion intensity differences for more accurate quantification of C=C regioisomeric lipids. We also plan to establish safe, efficient, and robust OzNOxESI on newer generation ion sources, making this capability available on newer mass spectrometers. In addition, we plan to develop a software suite to manage all data processing needs in lipid analysis, making it a one-stop solution for identification and quantification of lipids with structural details at the C=C regioisomer level. To achieve these goals, lipid standards and lipid extracts from cell lines, animal tissues, and human plasma will be thoroughly investigated as representative biological samples. The outcomes of this project can democratize advanced lipidomics capabilities and expedite the investigation of lipid unsaturation in biology and disease.
Grant Summary
Cost-effective workflow for efficient unsaturated lipidomics enabled by a novel ion chemistry is a NIGMS - National Institute of General Medical Sciences grant providing up to $1.3M for university, nonprofit, healthcare org. Applications are due 2030-03-31 (open). Check eligibility and apply with FindGrants.
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Up to $1.3M
2030-03-31
- 1Confirm your organization is eligible for Cost-effective workflow for efficient unsaturated lipidomics enabled by a novel ion chemistry from NIGMS - National Institute of General Medical Sciences, checking organization type, location, and any population or project requirements.
- 2Gather the required documents and information, including your organization details, project plan, and budget figures.
- 3Draft your application narrative and budget addressing the funder's priorities and review criteria. FindGrants can draft each section for you to review and edit.
- 4Review every section against the requirements checklist, then export a submission-ready application pack and submit it to NIGMS - National Institute of General Medical Sciences before the deadline.
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Cost-effective workflow for efficient unsaturated lipidomics enabled by a novel ion chemistry: Frequently Asked Questions
Who is eligible for the Cost-effective workflow for efficient unsaturated lipidomics enabled by a novel ion chemistry?
Cost-effective workflow for efficient unsaturated lipidomics enabled by a novel ion chemistry is offered by NIGMS - National Institute of General Medical Sciences and is generally open to university, nonprofit, healthcare org. It is open to organizations nationwide unless the funder specifies otherwise. Review the specific eligibility terms before applying, since funders set their own requirements around organization type, location, and the population or project being served.
How much funding does the Cost-effective workflow for efficient unsaturated lipidomics enabled by a novel ion chemistry provide?
Cost-effective workflow for efficient unsaturated lipidomics enabled by a novel ion chemistry provides up to $1.3M per award from NIGMS - National Institute of General Medical Sciences. Actual award sizes depend on the scope of your project, available program funds, and the number of applicants, so build a budget that reflects realistic, allowable costs rather than the maximum figure.
When is the Cost-effective workflow for efficient unsaturated lipidomics enabled by a novel ion chemistry deadline?
Applications for Cost-effective workflow for efficient unsaturated lipidomics enabled by a novel ion chemistry are due 2030-03-31 (open). Because deadlines can change, verify the date with the funder, NIGMS - National Institute of General Medical Sciences, and give yourself enough time to prepare a complete, competitive application before the close date.
How do you apply for the Cost-effective workflow for efficient unsaturated lipidomics enabled by a novel ion chemistry?
To apply for Cost-effective workflow for efficient unsaturated lipidomics enabled by a novel ion chemistry, confirm your eligibility, gather the required documents, and prepare a narrative and budget that address the funder's priorities. FindGrants guides you step by step and can draft each section, then exports a submission-ready application pack for this grant from NIGMS - National Institute of General Medical Sciences.