Control of transcriptional integrity by Integrator and PP2A
About This Grant
Project Summary Objective: The overall goals of this application are understanding the molecular basis of RNA Polymerase II (RNAPII) pausing and pause-release and determining how disease-associated mutations in the PP2A phosphatase reverse the pausing balance to promote transcriptional elongation. Proposed research: The mammalian RNAPII transcription cycle is regulated by cyclin-dependent kinases (CDKs) that target the catalytic core of RNAPII and many of its co-factors. We recently identified Int-PP2A, a module of the RNAPII-associated Integrator complex that recruits the PP2A phosphatase to active genes to oppose CDK9 activity. We demonstrated that Int-PP2A targets Ser-2 residues at the C terminal domain (CTD) of RNAPII as well as DSIF/Spt5. While our work revealed that phosphatases are recruited co-transcriptionally in mammalian cells to promote pausing, it remains unknown how Int-PP2A’s activity is established at the proximal promoter and how it is modulated. Here we propose to: a) determine how Int-PP2A establishes paused RNAPII via the INTS3 subunit; b) determine the role of PP2A carboxy-methylation in transcriptional regulation; c) determine the effect of mutant PP2A on transcription in models of neuronal differentiation.
Grant Summary
Control of transcriptional integrity by Integrator and PP2A is a NIGMS - National Institute of General Medical Sciences grant providing up to $592K for university, nonprofit, healthcare org. Applications are due 2031-03-31 (open). Check eligibility and apply with FindGrants.
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Eligibility
How to Apply
Up to $592K
2031-03-31
- 1Confirm your organization is eligible for Control of transcriptional integrity by Integrator and PP2A from NIGMS - National Institute of General Medical Sciences, checking organization type, location, and any population or project requirements.
- 2Gather the required documents and information, including your organization details, project plan, and budget figures.
- 3Draft your application narrative and budget addressing the funder's priorities and review criteria. FindGrants can draft each section for you to review and edit.
- 4Review every section against the requirements checklist, then export a submission-ready application pack and submit it to NIGMS - National Institute of General Medical Sciences before the deadline.
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Control of transcriptional integrity by Integrator and PP2A: Frequently Asked Questions
Who is eligible for the Control of transcriptional integrity by Integrator and PP2A?
Control of transcriptional integrity by Integrator and PP2A is offered by NIGMS - National Institute of General Medical Sciences and is generally open to university, nonprofit, healthcare org. It is open to organizations nationwide unless the funder specifies otherwise. Review the specific eligibility terms before applying, since funders set their own requirements around organization type, location, and the population or project being served.
How much funding does the Control of transcriptional integrity by Integrator and PP2A provide?
Control of transcriptional integrity by Integrator and PP2A provides up to $592K per award from NIGMS - National Institute of General Medical Sciences. Actual award sizes depend on the scope of your project, available program funds, and the number of applicants, so build a budget that reflects realistic, allowable costs rather than the maximum figure.
When is the Control of transcriptional integrity by Integrator and PP2A deadline?
Applications for Control of transcriptional integrity by Integrator and PP2A are due 2031-03-31 (open). Because deadlines can change, verify the date with the funder, NIGMS - National Institute of General Medical Sciences, and give yourself enough time to prepare a complete, competitive application before the close date.
How do you apply for the Control of transcriptional integrity by Integrator and PP2A?
To apply for Control of transcriptional integrity by Integrator and PP2A, confirm your eligibility, gather the required documents, and prepare a narrative and budget that address the funder's priorities. FindGrants guides you step by step and can draft each section, then exports a submission-ready application pack for this grant from NIGMS - National Institute of General Medical Sciences.