Development of an immunocompetent colon chip to model Crohn's Disease
NIDDK - National Institute of Diabetes and Digestive and Kidney Diseases
About This Grant
PROJECT SUMMARY/ABSTRACT The goal of this proposal is to develop an ex vivo experimental platform to enable novel investigations into the roles of the intestinal lumenal and basal microenvironment on the origins of Crohn's Disease (CD). CD is a chronic, idiopathic inflammatory disease of the intestines whose incidence and prevalence are increasing in the U.S. and globally. The disease has a disproportionate impact on the health and well-being of CD patients as well as significant use of health care resources. CD is thought to result from a breakdown of immune tolerance due to a compromised mucus hydrogel and/or epithelial cell barrier function early in the disease, allowing the inappropriate ingress of lumenal microbiota and their metabolites into the basal tissue microenvironment. Prior experimental models of CD are limited by the use of non-human or tumor cells that do not reflect human intestinal physiology, organoid models that do not fully mimic tissue microarchitecture and cell organization, or monolayer systems on stiff surfaces that lead to stem/proliferative cell loss and altered differentiated cell properties. To resolve these limitations, an interdisciplinary collaboration with expertise in advanced bioengineered intestinal platforms, immunology, and clinical gastroenterology has been formed to construct a 3D intestinal platform incorporating primary intestinal and immune cells to support a microenvironment that correctly mimics the colon in health and Crohn’s disease. A physiologic patterned mucus hydrogel will act as a protective barrier and lubricating surface along the lumenal epithelial cell surface. A magnetic fecal surrogate comprised of cellulose and colonic bacteria will recreate the properties of colonic fecal material and its biophysical and biochemical interactions with the lumenal epithelial surface. Collectively these advances in tissue engineering of a humanized colon-on-chip will create an accurate biointerface to produce a powerful ex vivo system to investigate the complex interplay of the intestinal epithelium, mucus hydrogel, fecal microbes/biomatrix and immune cells in health and CD. As a cell source, the project will take advantage of an extensive biobank of over 1000 HLA-typed normal donors and CD patients comprised of paired primary intestinal immune cells and colonic epithelium established at the Benaroya Institute. These specimens will enable the use of autologous tissues to avoid the confounding effects of allogeneic immune reactions.
Grant Summary
Development of an immunocompetent colon chip to model Crohn's Disease is a NIDDK - National Institute of Diabetes and Digestive and Kidney Diseases grant providing up to $826K for university, nonprofit, healthcare org. Applications are due 2030-01-31 (open). Check eligibility and apply with FindGrants.
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Focus Areas
Eligibility
How to Apply
Up to $826K
2030-01-31
- 1Confirm your organization is eligible for Development of an immunocompetent colon chip to model Crohn's Disease from NIDDK - National Institute of Diabetes and Digestive and Kidney Diseases, checking organization type, location, and any population or project requirements.
- 2Gather the required documents and information, including your organization details, project plan, and budget figures.
- 3Draft your application narrative and budget addressing the funder's priorities and review criteria. FindGrants can draft each section for you to review and edit.
- 4Review every section against the requirements checklist, then export a submission-ready application pack and submit it to NIDDK - National Institute of Diabetes and Digestive and Kidney Diseases before the deadline.
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Development of an immunocompetent colon chip to model Crohn's Disease: Frequently Asked Questions
Who is eligible for the Development of an immunocompetent colon chip to model Crohn's Disease?
Development of an immunocompetent colon chip to model Crohn's Disease is offered by NIDDK - National Institute of Diabetes and Digestive and Kidney Diseases and is generally open to university, nonprofit, healthcare org. It is open to organizations nationwide unless the funder specifies otherwise. Review the specific eligibility terms before applying, since funders set their own requirements around organization type, location, and the population or project being served.
How much funding does the Development of an immunocompetent colon chip to model Crohn's Disease provide?
Development of an immunocompetent colon chip to model Crohn's Disease provides up to $826K per award from NIDDK - National Institute of Diabetes and Digestive and Kidney Diseases. Actual award sizes depend on the scope of your project, available program funds, and the number of applicants, so build a budget that reflects realistic, allowable costs rather than the maximum figure.
When is the Development of an immunocompetent colon chip to model Crohn's Disease deadline?
Applications for Development of an immunocompetent colon chip to model Crohn's Disease are due 2030-01-31 (open). Because deadlines can change, verify the date with the funder, NIDDK - National Institute of Diabetes and Digestive and Kidney Diseases, and give yourself enough time to prepare a complete, competitive application before the close date.
How do you apply for the Development of an immunocompetent colon chip to model Crohn's Disease?
To apply for Development of an immunocompetent colon chip to model Crohn's Disease, confirm your eligibility, gather the required documents, and prepare a narrative and budget that address the funder's priorities. FindGrants guides you step by step and can draft each section, then exports a submission-ready application pack for this grant from NIDDK - National Institute of Diabetes and Digestive and Kidney Diseases.