Impact of opioid use disorder on mitochondrial dysfunction among antiretroviral therapy-receiving aging people with HIV.
NIDA - National Institute on Drug Abuse
About This Grant
Use of antiretroviral therapy (ART) has considerably increased life expectancy for people with HIV (PWH) resulting in increased risk of age-related non-AIDS-defining health burden, including inflammaging, a low-grade chronic proinflammatory immune response, among the older population of PWH compared to the general population. A large proportion of PWH are often found to be addicted to synthetic or non-synthetic opioids, resulting in opioid use disorder (OUD), which can also add to proinflammatory immune response. However, little is known about the consequences of long-term ART and OUD on inflammaging in this aging PWH. Interestingly, a common denominator for aging, HIV and OUD is that they all cause mitochondrial dysfunction in immune cells as indicated by various previous reports. Mitochondria are highly dynamic organelles, essential for cellular metabolism, stress responses and homeostasis maintenance and are considered as the hub for biochemical processes including oxidative phosphorylation (oxphos), adenosine triphosphate (ATP) production, intracellular reactive oxygen species (ROS) generation, fatty acid synthesis and calcium (Ca2+) homeostasis. Our previously published data have shown that mitochondrial ROS increased with mitochondrial depolarization in people with HIV. Moreover, our exciting preliminary data indicated that mitochondrial ROS in immune cells is further increased in older PWH with age ≥50years compared to younger PWH with age <50 years. In addition, PWH with OUD had more mitochondrial ROS+ and reduced mitochondrial membrane potential in immune cells compared to PWH without OUD. Also, we found increased inflammatory immune markers in plasma of PWH with OUD. In view of this evidence, we hypothesize that OUD aggravates inflammatory immune response by increased mitochondrial dysfunctions in aging PWH. To test our hypothesis, we propose to evaluate the extent of OUD induced mitochondrial dysfunction in aging PWH, by studying mitochondrial mass, mitochondrial superoxide levels, mitochondrial membrane potential, mitochondrial fusion/fission, mitophagy, expression of mitochondrial stress response genes, oxidative phosphorylation and mitochondrial NADH levels in peripheral blood mononuclear cells (PBMC) of ART-treated PWH of age ≥50years, with or without OUD. In addition, we will identify cell specific molecular targets contributing to mitochondrial dysfunction, cellular senescence and inflammaging in PWH with OUD by transcriptomic analysis using single cell RNAseq. Finally, we will investigate if treatment with ROS a scavenger or a NAD+ booster can improve mitochondrial function in immune cells and reduce proinflammatory immune response in aging PWH with OUD. This proposal aims to advance the foundational knowledge about the role of mitochondria in the age-related altered immune cell function in older ART receiving PWH with OUD, which could be leveraged to develop targeted therapies and improve outcomes in people aging with HIV, opioid use and addiction.
Grant Summary
Impact of opioid use disorder on mitochondrial dysfunction among antiretroviral therapy-receiving aging people with HIV. is a NIDA - National Institute on Drug Abuse grant providing up to $686K for university, nonprofit, healthcare org. Applications are due 2030-12-31 (open). Check eligibility and apply with FindGrants.
Focus Areas
Eligibility
How to Apply
Up to $686K
2030-12-31
- 1Confirm your organization is eligible for Impact of opioid use disorder on mitochondrial dysfunction among antiretroviral therapy-receiving aging people with HIV. from NIDA - National Institute on Drug Abuse, checking organization type, location, and any population or project requirements.
- 2Gather the required documents and information, including your organization details, project plan, and budget figures.
- 3Draft your application narrative and budget addressing the funder's priorities and review criteria. FindGrants can draft each section for you to review and edit.
- 4Review every section against the requirements checklist, then export a submission-ready application pack and submit it to NIDA - National Institute on Drug Abuse before the deadline.
Don't want to draft it yourself?
We'll draft the complete application against NIDA - National Institute on Drug Abuse's requirements, run a quality review, and email you a submission-ready PDF plus an editable Word doc within 5 business days. Most orders deliver in 24-48 hours. Flat $399, any grant size.
AI Requirement Analysis
Detailed requirements not yet analyzed
Have the NOFO? Paste it below for AI-powered requirement analysis.
Impact of opioid use disorder on mitochondrial dysfunction among antiretroviral therapy-receiving aging people with HIV.: Frequently Asked Questions
Who is eligible for the Impact of opioid use disorder on mitochondrial dysfunction among antiretroviral therapy-receiving aging people with HIV.?
Impact of opioid use disorder on mitochondrial dysfunction among antiretroviral therapy-receiving aging people with HIV. is offered by NIDA - National Institute on Drug Abuse and is generally open to university, nonprofit, healthcare org. It is open to organizations nationwide unless the funder specifies otherwise. Review the specific eligibility terms before applying, since funders set their own requirements around organization type, location, and the population or project being served.
How much funding does the Impact of opioid use disorder on mitochondrial dysfunction among antiretroviral therapy-receiving aging people with HIV. provide?
Impact of opioid use disorder on mitochondrial dysfunction among antiretroviral therapy-receiving aging people with HIV. provides up to $686K per award from NIDA - National Institute on Drug Abuse. Actual award sizes depend on the scope of your project, available program funds, and the number of applicants, so build a budget that reflects realistic, allowable costs rather than the maximum figure.
When is the Impact of opioid use disorder on mitochondrial dysfunction among antiretroviral therapy-receiving aging people with HIV. deadline?
Applications for Impact of opioid use disorder on mitochondrial dysfunction among antiretroviral therapy-receiving aging people with HIV. are due 2030-12-31 (open). Because deadlines can change, verify the date with the funder, NIDA - National Institute on Drug Abuse, and give yourself enough time to prepare a complete, competitive application before the close date.
How do you apply for the Impact of opioid use disorder on mitochondrial dysfunction among antiretroviral therapy-receiving aging people with HIV.?
To apply for Impact of opioid use disorder on mitochondrial dysfunction among antiretroviral therapy-receiving aging people with HIV., confirm your eligibility, gather the required documents, and prepare a narrative and budget that address the funder's priorities. FindGrants guides you step by step and can draft each section, then exports a submission-ready application pack for this grant from NIDA - National Institute on Drug Abuse.