Novel methods for the design, analysis and implementation of ELISpot experiments
NCI - National Cancer Institute
About This Grant
The enzyme-linked immunospot (ELIspot) assay is used to quantify the number of cytokine secreting cells and is the focus of numerous basic, clinical and translational studies. The results from an ELIspot assay can be used as an outcome in early stage trials for comparing the immunogenicity of different therapies or as a short-term indicator of long-term clinical benefit of an immunotherapy. Despite its widespread use, there is little consistency in how ELIspot data are analyzed. Numerous proposals have been made that define immunologic response as a dichotomous endpoint (response/no response) based on empirical rules for classifying response as a combination of ELIspot count and negative control values. The multiplicity of definitions makes it difficult, if not impossible, to compare results across studies. Moreover, the use of the ELIspot results as a continuous endpoint should have greater statistical power than a dichotomous endpoint and may allow researchers greater opportunity to discover how patterns over time are associated with long-term outcome. This project proposes a method for analyzing ELIspot data as a continuous measure that adjusts ELIspot count for negative and positive controls. We have data from numerous clinical studies that we can use as a basis for comparing the statistical power of our method with existing methods. Furthermore, we will assess whether patterns of immunologic response are associated with long-term clinical benefit. In the process of improving the analysis of data from ELIspot assays, we will propose several innovative statistical methods that can be used in a wide range of applications.
Grant Summary
Novel methods for the design, analysis and implementation of ELISpot experiments is a NCI - National Cancer Institute grant providing up to $484K for university, nonprofit, healthcare org. Applications are due 2030-04-30 (open). Check eligibility and apply with FindGrants.
Focus Areas
Eligibility
How to Apply
Up to $484K
2030-04-30
- 1Confirm your organization is eligible for Novel methods for the design, analysis and implementation of ELISpot experiments from NCI - National Cancer Institute, checking organization type, location, and any population or project requirements.
- 2Gather the required documents and information, including your organization details, project plan, and budget figures.
- 3Draft your application narrative and budget addressing the funder's priorities and review criteria. FindGrants can draft each section for you to review and edit.
- 4Review every section against the requirements checklist, then export a submission-ready application pack and submit it to NCI - National Cancer Institute before the deadline.
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Novel methods for the design, analysis and implementation of ELISpot experiments: Frequently Asked Questions
Who is eligible for the Novel methods for the design, analysis and implementation of ELISpot experiments?
Novel methods for the design, analysis and implementation of ELISpot experiments is offered by NCI - National Cancer Institute and is generally open to university, nonprofit, healthcare org. It is open to organizations nationwide unless the funder specifies otherwise. Review the specific eligibility terms before applying, since funders set their own requirements around organization type, location, and the population or project being served.
How much funding does the Novel methods for the design, analysis and implementation of ELISpot experiments provide?
Novel methods for the design, analysis and implementation of ELISpot experiments provides up to $484K per award from NCI - National Cancer Institute. Actual award sizes depend on the scope of your project, available program funds, and the number of applicants, so build a budget that reflects realistic, allowable costs rather than the maximum figure.
When is the Novel methods for the design, analysis and implementation of ELISpot experiments deadline?
Applications for Novel methods for the design, analysis and implementation of ELISpot experiments are due 2030-04-30 (open). Because deadlines can change, verify the date with the funder, NCI - National Cancer Institute, and give yourself enough time to prepare a complete, competitive application before the close date.
How do you apply for the Novel methods for the design, analysis and implementation of ELISpot experiments?
To apply for Novel methods for the design, analysis and implementation of ELISpot experiments, confirm your eligibility, gather the required documents, and prepare a narrative and budget that address the funder's priorities. FindGrants guides you step by step and can draft each section, then exports a submission-ready application pack for this grant from NCI - National Cancer Institute.