Control of host invasion by protein complexes conserved across apicomplexan parasites
NIAID - National Institute of Allergy and Infectious Diseases
About This Grant
7. PROJECT SUMMARY/ABSTRACT How apicomplexan parasites recognize host cells to invade them remains a mystery. Since these parasites only replicate intracellularly, invading host cells is essential to their survival. Invasion relies on the coordinated secretion of proteins from two distinct types of organelles: micronemes and rhoptries. Rhoptries are exclusively discharged upon host-cell contact; however, the molecular events that trigger this process remain completely unknown. Understanding the factors required for rhoptry discharge can help us uncover the basis of host-cell recognition. Two conserved microneme complexes—the CLAMP and CRMP complexes—have recently been shown by the Lourido and Lebrun labs to be required for rhoptry discharge, making them likely mediators of host-cell recognition. Our preliminary studies have identified N-glycosylation pathways as key host requirements for rhoptry discharge. Loss of host N-glycosylation significantly reduced rhoptry discharge, while buffering any additional effect from knocking down the CLAMP or CRMP complexes. Based on these observations, we hypothesize that the CLAMP and CRMP complexes recognize host cells through N-glycans to mediate rhoptry discharge. This proposal brings together the two teams that discovered these critical complexes to examine how they recognize host N-glycosylation and mediate rhoptry discharge. Aim 1 will determine the glycan specificity of the CLAMP and CRMP complexes through traditional or liquid glycan arrays. Aim 2 will characterize how N-glycosylation alters the various stages of invasion using high-speed live-cell imaging to dissect the multi-stage invasion process and pinpoint defects to rhoptry discharge. Aim 3 will characterize the signaling events triggered by host-cell recognition through phosphoproteomics and proximity-labeling. Taken together the proposed experiments will uncover how apicomplexans respond to host contact to discharge rhoptries and initiate invasion. Beyond its fundamental importance, this information can be used to design inhibitors of host cell entry that would block parasite infection.
Grant Summary
Control of host invasion by protein complexes conserved across apicomplexan parasites is a NIAID - National Institute of Allergy and Infectious Diseases grant providing up to $900K for university, nonprofit, healthcare org. Applications are due 2031-01-31 (open). Check eligibility and apply with FindGrants.
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Up to $900K
2031-01-31
- 1Confirm your organization is eligible for Control of host invasion by protein complexes conserved across apicomplexan parasites from NIAID - National Institute of Allergy and Infectious Diseases, checking organization type, location, and any population or project requirements.
- 2Gather the required documents and information, including your organization details, project plan, and budget figures.
- 3Draft your application narrative and budget addressing the funder's priorities and review criteria. FindGrants can draft each section for you to review and edit.
- 4Review every section against the requirements checklist, then export a submission-ready application pack and submit it to NIAID - National Institute of Allergy and Infectious Diseases before the deadline.
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Control of host invasion by protein complexes conserved across apicomplexan parasites: Frequently Asked Questions
Who is eligible for the Control of host invasion by protein complexes conserved across apicomplexan parasites?
Control of host invasion by protein complexes conserved across apicomplexan parasites is offered by NIAID - National Institute of Allergy and Infectious Diseases and is generally open to university, nonprofit, healthcare org. It is open to organizations nationwide unless the funder specifies otherwise. Review the specific eligibility terms before applying, since funders set their own requirements around organization type, location, and the population or project being served.
How much funding does the Control of host invasion by protein complexes conserved across apicomplexan parasites provide?
Control of host invasion by protein complexes conserved across apicomplexan parasites provides up to $900K per award from NIAID - National Institute of Allergy and Infectious Diseases. Actual award sizes depend on the scope of your project, available program funds, and the number of applicants, so build a budget that reflects realistic, allowable costs rather than the maximum figure.
When is the Control of host invasion by protein complexes conserved across apicomplexan parasites deadline?
Applications for Control of host invasion by protein complexes conserved across apicomplexan parasites are due 2031-01-31 (open). Because deadlines can change, verify the date with the funder, NIAID - National Institute of Allergy and Infectious Diseases, and give yourself enough time to prepare a complete, competitive application before the close date.
How do you apply for the Control of host invasion by protein complexes conserved across apicomplexan parasites?
To apply for Control of host invasion by protein complexes conserved across apicomplexan parasites, confirm your eligibility, gather the required documents, and prepare a narrative and budget that address the funder's priorities. FindGrants guides you step by step and can draft each section, then exports a submission-ready application pack for this grant from NIAID - National Institute of Allergy and Infectious Diseases.