Novel mechanisms to inactivate bacterial adhesins
NIAID - National Institute of Allergy and Infectious Diseases
About This Grant
ABSTRACT In this project, we aim to unravel the structural basis of a novel parasteric mechanism of inhibition of bacterial adhesion. This mechanism was discovered against the main adhesin of uropathogenic E. coli—the mannose- specific fimbrial lectin FimH—which is a critical urovirulence factor and a major vaccine target. FimH is an allosteric protein that exists in two conformations: a low-affinity “inactive” state and a high-affinity “active” state. We found that these alternative conformations of FimH are recognized by different monoclonal antibodies, which have distinct inhibitory effects on the adhesin’s ability to bind mannose receptors. Cryo-EM structures of these antibodies in complex with FimH have been recently resolved, and this information will be utilized to characterize the ability of certain antibodies and de novo-designed miniproteins to mediate parasteric inhibition. A unique hallmark of this type of inhibitor is its ability to both block and reverse bacterial adhesion and biofilm formation. We will further define the structural details of the interactions between FimH and parasteric inhibitors and optimize these inhibitors before testing their efficacy in protecting against UTIs using a mouse model of passive intra-peritoneal immunization. Completion of these aims will facilitate the development of conceptually novel therapeutics against uropathogenic E. coli and other human pathogens.
Grant Summary
Novel mechanisms to inactivate bacterial adhesins is a NIAID - National Institute of Allergy and Infectious Diseases grant providing up to $777K for university, nonprofit, healthcare org. Applications are due 2031-04-30 (open). Check eligibility and apply with FindGrants.
Focus Areas
Eligibility
How to Apply
Up to $777K
2031-04-30
- 1Confirm your organization is eligible for Novel mechanisms to inactivate bacterial adhesins from NIAID - National Institute of Allergy and Infectious Diseases, checking organization type, location, and any population or project requirements.
- 2Gather the required documents and information, including your organization details, project plan, and budget figures.
- 3Draft your application narrative and budget addressing the funder's priorities and review criteria. FindGrants can draft each section for you to review and edit.
- 4Review every section against the requirements checklist, then export a submission-ready application pack and submit it to NIAID - National Institute of Allergy and Infectious Diseases before the deadline.
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Novel mechanisms to inactivate bacterial adhesins: Frequently Asked Questions
Who is eligible for the Novel mechanisms to inactivate bacterial adhesins?
Novel mechanisms to inactivate bacterial adhesins is offered by NIAID - National Institute of Allergy and Infectious Diseases and is generally open to university, nonprofit, healthcare org. It is open to organizations nationwide unless the funder specifies otherwise. Review the specific eligibility terms before applying, since funders set their own requirements around organization type, location, and the population or project being served.
How much funding does the Novel mechanisms to inactivate bacterial adhesins provide?
Novel mechanisms to inactivate bacterial adhesins provides up to $777K per award from NIAID - National Institute of Allergy and Infectious Diseases. Actual award sizes depend on the scope of your project, available program funds, and the number of applicants, so build a budget that reflects realistic, allowable costs rather than the maximum figure.
When is the Novel mechanisms to inactivate bacterial adhesins deadline?
Applications for Novel mechanisms to inactivate bacterial adhesins are due 2031-04-30 (open). Because deadlines can change, verify the date with the funder, NIAID - National Institute of Allergy and Infectious Diseases, and give yourself enough time to prepare a complete, competitive application before the close date.
How do you apply for the Novel mechanisms to inactivate bacterial adhesins?
To apply for Novel mechanisms to inactivate bacterial adhesins, confirm your eligibility, gather the required documents, and prepare a narrative and budget that address the funder's priorities. FindGrants guides you step by step and can draft each section, then exports a submission-ready application pack for this grant from NIAID - National Institute of Allergy and Infectious Diseases.