Using Metaproteomics To Determine How Sources Of Dietary Protein And Fiber Interact To Alter Gut Microbiome Function In The Context Of Inflammation
NIDDK - National Institute of Diabetes and Digestive and Kidney Diseases
About This Grant
PROJECT SUMMARY/ABSTRACT Inflammatory bowel diseases (IBD) represent a major quality of life burden due to persistent symptoms, including abdominal pain, diarrhea, malnutrition, weight loss, and depression. Multiple factors cause IBD including lifestyle choices (e.g., poor diet), genetics, and the gut microbiota. To improve the detection and treatment of IBD there exists a critical need to increase understanding of the reciprocal relationship between diet, gut microbiota function, and inflammation. The candidate, J. Alfredo Blakeley-Ruiz, is a PhD scientist with extensive expertise in the use of the multi-omics approaches metagenomics and metaproteomics. Using integrated metagenomics-metaproteomics it is possible to observe overall shifts in microbial metabolism, while also measuring the responses of the immune system through the quantification of host proteins. Thus, integrated metagenomics-metaproteomics represents a powerful tool for untangling the relationship between diet, gut microbiota function, and inflammation. The candidate's long-term goal is to develop metaproteomics tools to identify the dietary components and microbiome functions that contribute to inflammation in IBD patients, so that dietary recommendations can be made to ameliorate inflammation in patients suffering from IBD and other intestinal diseases. This proposal builds on the candidate's expertise by providing training in the skills needed to connect his metaproteomic data to the host's intestinal immune response. The protected time provided by this proposal will allow the candidate to focus on training in (1) techniques for profiling GI physiology, (2) translatable models of IBD and the evaluation of their pathology, and (3) the husbandry of in vivo models. This training will allow the candidate to investigate the mechanisms behind how diet induced changes in microbiome function negatively impact the immune system leading to increased IBD pathology. The central hypothesis is that the digestibility and glycan conjugates of dietary proteins synergize with complex carbohydrates (e.g., starch and fermentable fiber) to change gut microbiome metabolism. This affects susceptibility to IBD via microbial metabolism mediated changes in the local immune cell landscape and colonic barrier integrity. This hypothesis will be investigated by feeding wild type and IL-10-/- mice with conventional or humanized microbiomes different combinations of fiber and protein diets, evaluating their colonic immune cell landscape, intestinal barrier integrity, and IBD pathology. These readouts will be linked to microbial and host protein quantities of metaproteomes to tease apart the mechanisms behind how diet-induced changes in microbiome function lead to IBD. NCSU is the perfect institutional environment for this research and training plan. The College of Veterinary Medicine gives access to the clinician scientists, core facilities, animal research facilities (NCSU gnotobiotic core), and didactics needed to fulfill the training plan of this proposal. NCSU has state of the art mass spectrometry (METRIC) and sequencing facilities (Genomics Sciences Laboratory) needed to conduct metagenomic and metaproteomic measurements.
Grant Summary
Using Metaproteomics To Determine How Sources Of Dietary Protein And Fiber Interact To Alter Gut Microbiome Function In The Context Of Inflammation is a NIDDK - National Institute of Diabetes and Digestive and Kidney Diseases grant providing up to $166K for university, nonprofit, healthcare org. Applications are due 2031-07-31 (open). Check eligibility and apply with FindGrants.
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Up to $166K
2031-07-31
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Using Metaproteomics To Determine How Sources Of Dietary Protein And Fiber Interact To Alter Gut Microbiome Function In The Context Of Inflammation: Frequently Asked Questions
Who is eligible for the Using Metaproteomics To Determine How Sources Of Dietary Protein And Fiber Interact To Alter Gut Microbiome Function In The Context Of Inflammation?
Using Metaproteomics To Determine How Sources Of Dietary Protein And Fiber Interact To Alter Gut Microbiome Function In The Context Of Inflammation is offered by NIDDK - National Institute of Diabetes and Digestive and Kidney Diseases and is generally open to university, nonprofit, healthcare org. It is open to organizations nationwide unless the funder specifies otherwise. Review the specific eligibility terms before applying, since funders set their own requirements around organization type, location, and the population or project being served.
How much funding does the Using Metaproteomics To Determine How Sources Of Dietary Protein And Fiber Interact To Alter Gut Microbiome Function In The Context Of Inflammation provide?
Using Metaproteomics To Determine How Sources Of Dietary Protein And Fiber Interact To Alter Gut Microbiome Function In The Context Of Inflammation provides up to $166K per award from NIDDK - National Institute of Diabetes and Digestive and Kidney Diseases. Actual award sizes depend on the scope of your project, available program funds, and the number of applicants, so build a budget that reflects realistic, allowable costs rather than the maximum figure.
When is the Using Metaproteomics To Determine How Sources Of Dietary Protein And Fiber Interact To Alter Gut Microbiome Function In The Context Of Inflammation deadline?
Applications for Using Metaproteomics To Determine How Sources Of Dietary Protein And Fiber Interact To Alter Gut Microbiome Function In The Context Of Inflammation are due 2031-07-31 (open). Because deadlines can change, verify the date with the funder, NIDDK - National Institute of Diabetes and Digestive and Kidney Diseases, and give yourself enough time to prepare a complete, competitive application before the close date.
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To apply for Using Metaproteomics To Determine How Sources Of Dietary Protein And Fiber Interact To Alter Gut Microbiome Function In The Context Of Inflammation, confirm your eligibility, gather the required documents, and prepare a narrative and budget that address the funder's priorities. FindGrants guides you step by step and can draft each section, then exports a submission-ready application pack for this grant from NIDDK - National Institute of Diabetes and Digestive and Kidney Diseases.