Mechanisms of endosomal dysfunction in alcohol-related steatosis and liver injury
About This Grant
Project Summary/Abstract My professional career goal is to conduct independent research in life sciences defining complex biological systems, including and specifically alcohol-associated liver disease (ALD). ALD is a progressive disease that can lead to irreversible and fatal liver damage in the form of inflammation, fibrosis, cirrhosis, and cancer. Unfortunately, therapeutic treatments for ALD are limited. This is in part due to the lack of understanding of ALD progression at the cellular level. The onset of ALD is represented by a reversible steatotic liver stage where lipid droplets (LDs) excessively accumulate within hepatocytes, the highly metabolizing cell type of the liver. This observation suggests chronic alcohol consumption prevents the breakdown of LDs. Previous studies have shown alcohol disrupts endocytic vesicle trafficking proteins, including Rab5, and LD catabolism; however, these cellular mechanisms of perturbation are poorly understood. Therefore, this proposal aims to elucidate the molecular mechanisms in which alcohol affects Rab5 and endocytic trafficking during ALD progression. Preliminary studies show alcohol disrupts Rab5 subcellular localization without affecting Rab5 activity. Altogether, the central hypothesis is alcohol disrupts Rab5 prenylation, a post-translational modification, to drive its altered localization. This will be tested by two specific aims. Aim 1 will define the impact of alcohol on Rab5 subcellular localization and remodeling of the endosome proteome. Aim 2 will determine the impact of alcohol on global changes in protein prenylation (prenyl-proteome) including Rab5. To accomplish these aims, I will acquire training in confocal microscopy and mass spectrometry. This new training will broaden my current skillsets regarding yeast cell culture and genetics, small molecule trafficking and signaling, the use and design of fluorescent biosensors, and inter-organelle communication. My strong advisory team, including my sponsor and co-sponsor Dr. Micah Schott and Dr. Carol Casey, respectively, one collaborator, and five other faculty have significant expertise in the proposed topics and the desired training objectives. The microscopy and mass spectrometry core facilities, and research environment at UNMC will enhance this research and training. Together, I will have the expertise and training needed to successfully accomplish these aims. The results gained from the proposed research will provide a mechanistic understanding of disrupted endocytic trafficking by alcohol in ALD.
Grant Summary
Mechanisms of endosomal dysfunction in alcohol-related steatosis and liver injury is a NIAAA - National Institute on Alcohol Abuse and Alcoholism grant providing up to $77K for university, nonprofit, healthcare org. Applications are due 2029-04-30 (open). Check eligibility and apply with FindGrants.
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How to Apply
Up to $77K
2029-04-30
- 1Confirm your organization is eligible for Mechanisms of endosomal dysfunction in alcohol-related steatosis and liver injury from NIAAA - National Institute on Alcohol Abuse and Alcoholism, checking organization type, location, and any population or project requirements.
- 2Gather the required documents and information, including your organization details, project plan, and budget figures.
- 3Draft your application narrative and budget addressing the funder's priorities and review criteria. FindGrants can draft each section for you to review and edit.
- 4Review every section against the requirements checklist, then export a submission-ready application pack and submit it to NIAAA - National Institute on Alcohol Abuse and Alcoholism before the deadline.
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Mechanisms of endosomal dysfunction in alcohol-related steatosis and liver injury: Frequently Asked Questions
Who is eligible for the Mechanisms of endosomal dysfunction in alcohol-related steatosis and liver injury?
Mechanisms of endosomal dysfunction in alcohol-related steatosis and liver injury is offered by NIAAA - National Institute on Alcohol Abuse and Alcoholism and is generally open to university, nonprofit, healthcare org. It is open to organizations nationwide unless the funder specifies otherwise. Review the specific eligibility terms before applying, since funders set their own requirements around organization type, location, and the population or project being served.
How much funding does the Mechanisms of endosomal dysfunction in alcohol-related steatosis and liver injury provide?
Mechanisms of endosomal dysfunction in alcohol-related steatosis and liver injury provides up to $77K per award from NIAAA - National Institute on Alcohol Abuse and Alcoholism. Actual award sizes depend on the scope of your project, available program funds, and the number of applicants, so build a budget that reflects realistic, allowable costs rather than the maximum figure.
When is the Mechanisms of endosomal dysfunction in alcohol-related steatosis and liver injury deadline?
Applications for Mechanisms of endosomal dysfunction in alcohol-related steatosis and liver injury are due 2029-04-30 (open). Because deadlines can change, verify the date with the funder, NIAAA - National Institute on Alcohol Abuse and Alcoholism, and give yourself enough time to prepare a complete, competitive application before the close date.
How do you apply for the Mechanisms of endosomal dysfunction in alcohol-related steatosis and liver injury?
To apply for Mechanisms of endosomal dysfunction in alcohol-related steatosis and liver injury, confirm your eligibility, gather the required documents, and prepare a narrative and budget that address the funder's priorities. FindGrants guides you step by step and can draft each section, then exports a submission-ready application pack for this grant from NIAAA - National Institute on Alcohol Abuse and Alcoholism.